1buj

From Proteopedia

(Difference between revisions)

Current revision

STRUCTURE OF BINASE IN SOLUTION

Structural highlights

1buj is a 1 chain structure with sequence from Bacillus intermedius. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RN_BACIN This is a purine-specific ribonuclease.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We present the spatial structure of binase, a small extracellular ribonuclease, derived from 1H-NMR* data in aqueous solution. The total of 20 structures were obtained via torsion angle dynamics using DYANA program with experimental NOE and hydrogen bond distance constraints and phi and chi1 dihedral angle constraints. The final structures were energy minimised with ECEPP/2 potential in FANTOM program. Binase consists of three alpha-helices in N-terminal part (residues 6-16, 26-32 and 41-44), five-stranded antiparallel beta-sheet in C-terminal part (residues 51-55, 70-75, 86-90, 94-99 and 104-108) and two-stranded parallel beta-sheet (residues 22-24 and 49-51). Three loops (residues 36-39, 56-67 and 76-83), which play significant role in biological functioning of binase, are flexible in solution. The differences between binase and barnase spatial structures in solution explain the differences in thermostability of binase, barnase and their hybrids.

Three-dimensional structure of binase in solution.,Reibarkh MYa, Nolde DE, Vasilieva LI, Bocharov EV, Shulga AA, Kirpichnikov MP, Arseniev AS FEBS Lett. 1998 Jul 17;431(2):250-4. PMID:9708913^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Reibarkh MYa, Nolde DE, Vasilieva LI, Bocharov EV, Shulga AA, Kirpichnikov MP, Arseniev AS. Three-dimensional structure of binase in solution. FEBS Lett. 1998 Jul 17;431(2):250-4. PMID:9708913

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1buj"

@@ Line 1: / Line 1: @@
 ==STRUCTURE OF BINASE IN SOLUTION==
-<StructureSection load='1buj' size='340' side='right'caption='[[1buj]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='1buj' size='340' side='right'caption='[[1buj]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1buj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/'bacillus_intermedius' 'bacillus intermedius']. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BUJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1buj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_intermedius Bacillus intermedius]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BUJ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1buj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1buj OCA], [https://pdbe.org/1buj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1buj RCSB], [https://www.ebi.ac.uk/pdbsum/1buj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1buj ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1buj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1buj OCA], [https://pdbe.org/1buj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1buj RCSB], [https://www.ebi.ac.uk/pdbsum/1buj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1buj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/RN_BACIN RN_BACIN]] This is a purine-specific ribonuclease.
+[https://www.uniprot.org/uniprot/RN_BACIN RN_BACIN] This is a purine-specific ribonuclease.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 36: / Line 37: @@
 [[Category: Bacillus intermedius]]
 [[Category: Large Structures]]
-[[Category: Arseniev, A S]]
+[[Category: Arseniev AS]]
-[[Category: Nolde, D E]]
+[[Category: Nolde DE]]
-[[Category: Reibarkh, M Y]]
+[[Category: Reibarkh MY]]
-[[Category: Vasilieva, L I]]
+[[Category: Vasilieva LI]]
-[[Category: Hydrolase]]
-[[Category: Microbial ribonuclease]]

1buj

From Proteopedia

Current revision

STRUCTURE OF BINASE IN SOLUTION

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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