7fbt

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(New page: '''Unreleased structure''' The entry 7fbt is ON HOLD Authors: Jiang, Z.Q., Hu, S.Q., Zhu, Q., Liu, Y.C., Ma, J.W., Yan, Q.J., Gao, Y.G., Yang, S.Q. Description: Crystal structure of ch...)
Current revision (17:13, 29 November 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 7fbt is ON HOLD
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==Crystal structure of chitinase (RmChi1) from Rhizomucor miehei (sp p32 2 1, MR)==
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<StructureSection load='7fbt' size='340' side='right'caption='[[7fbt]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7fbt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5xwq 5xwq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FBT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fbt OCA], [https://pdbe.org/7fbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fbt RCSB], [https://www.ebi.ac.uk/pdbsum/7fbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fbt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A3B6UEQ2_RHIMI A0A3B6UEQ2_RHIMI]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A chitinase gene (RmChiA) encoding 445 amino acid (aa) residues from a fungus Rhizomucor miehei was cloned and overexpressed in Escherichia coli. Two kinds of RmChiA crystal forms, with space groups P32 2 1 and P1, were obtained by sitting-drop vapor diffusion and the structures were determined by X-ray diffraction. The overall structure of RmChiA monomer, which is the first structure of bacterial-type chitinases from nonpathogenic fungi, adopts a canonical triosephosphate isomerase (TIM) barrel fold with two protruding chitinase insertion domains. RmChiA exhibited a unique NxDxE catalytical motif and a real active site tunnel structure, which are firstly found in GH family 18 chitinases. The motif had high structural homolog with the typical DxDxE motif in other GH family 18 chitinases. The tunnel is formed by two unusual long loops, containing 15 aa and 45 aa respectively, linked by a disulfide bond across the substrate-binding cleft. Mutation experiments found that opening the roof of tunnel structure increased the hydrolysis efficiency of RmChiA, but the thermostability of the mutants decreased. Moreover, the tunnel structure endowed RmChiA with the exo-chitinase character.
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Authors: Jiang, Z.Q., Hu, S.Q., Zhu, Q., Liu, Y.C., Ma, J.W., Yan, Q.J., Gao, Y.G., Yang, S.Q.
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Crystal structure of a chitinase (RmChiA) from the thermophilic fungus Rhizomucor miehei with a real active site tunnel.,Jiang Z, Hu S, Ma J, Liu Y, Qiao Z, Yan Q, Gao Y, Yang S Biochim Biophys Acta Proteins Proteom. 2021 Aug 4;1869(11):140709. doi:, 10.1016/j.bbapap.2021.140709. PMID:34358705<ref>PMID:34358705</ref>
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Description: Crystal structure of chitinase (RmChi1) from Rhizomucor miehei (sp p32 2 1, MR)
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Jiang, Z.Q]]
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<div class="pdbe-citations 7fbt" style="background-color:#fffaf0;"></div>
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[[Category: Gao, Y.G]]
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[[Category: Yang, S.Q]]
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==See Also==
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[[Category: Liu, Y.C]]
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*[[Chitinase 3D structures|Chitinase 3D structures]]
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[[Category: Yan, Q.J]]
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== References ==
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[[Category: Hu, S.Q]]
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<references/>
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[[Category: Ma, J.W]]
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__TOC__
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[[Category: Zhu, Q]]
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Rhizomucor miehei]]
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[[Category: Gao YG]]
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[[Category: Hu SQ]]
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[[Category: Jiang ZQ]]
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[[Category: Liu YC]]
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[[Category: Ma JW]]
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[[Category: Yan QJ]]
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[[Category: Yang SQ]]
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[[Category: Zhu Q]]

Current revision

Crystal structure of chitinase (RmChi1) from Rhizomucor miehei (sp p32 2 1, MR)

PDB ID 7fbt

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