7p5x
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Mycobacterial RNAP with transcriptional activator PafBC== | |
| + | <StructureSection load='7p5x' size='340' side='right'caption='[[7p5x]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7p5x]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P5X FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p5x OCA], [https://pdbe.org/7p5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p5x RCSB], [https://www.ebi.ac.uk/pdbsum/7p5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p5x ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RPOA_MYCS2 RPOA_MYCS2] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_00059]<ref>PMID:19926651</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Transcriptional activator PafBC is the key regulator of the mycobacterial DNA damage response and controls around 150 genes, including genes involved in the canonical SOS response, through an unknown molecular mechanism. Using a combination of biochemistry and cryo-electron microscopy, we demonstrate that PafBC in the presence of single-stranded DNA activates transcription by reprogramming the canonical -10 and -35 promoter specificity of RNA polymerase associated with the housekeeping sigma subunit. We determine the structure of this transcription initiation complex, revealing a unique mode of promoter recognition, which we term "sigma adaptation." PafBC inserts between DNA and sigma factor to mediate recognition of hybrid promoters lacking the -35 but featuring the canonical -10 and a PafBC-specific -26 element. Sigma adaptation may constitute a more general mechanism of transcriptional control in mycobacteria. | ||
| - | + | Transcriptional control of mycobacterial DNA damage response by sigma adaptation.,Muller AU, Kummer E, Schilling CM, Ban N, Weber-Ban E Sci Adv. 2021 Dec 3;7(49):eabl4064. doi: 10.1126/sciadv.abl4064. Epub 2021 Dec 1. PMID:34851662<ref>PMID:34851662</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7p5x" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | ||
| + | *[[Sigma factor 3D structures|Sigma factor 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mycolicibacterium smegmatis MC2 155]] | ||
| + | [[Category: Ban N]] | ||
| + | [[Category: Kummer E]] | ||
| + | [[Category: Mueller AU]] | ||
| + | [[Category: Schilling CM]] | ||
| + | [[Category: Weber-Ban E]] | ||
Current revision
Mycobacterial RNAP with transcriptional activator PafBC
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