1evx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:07, 7 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1evx.gif|left|200px]]
 
-
<!--
+
==APO CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE, I-PPOI==
-
The line below this paragraph, containing "STRUCTURE_1evx", creates the "Structure Box" on the page.
+
<StructureSection load='1evx' size='340' side='right'caption='[[1evx]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1evx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EVX FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-
{{STRUCTURE_1evx| PDB=1evx | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1evx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1evx OCA], [https://pdbe.org/1evx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1evx RCSB], [https://www.ebi.ac.uk/pdbsum/1evx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1evx ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.
-
'''APO CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE, I-PPOI'''
+
==See Also==
-
 
+
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The homing endonuclease I-PpoI severely bends its DNA target, resulting in significant deformations of the minor and major groove near the scissile phosphate groups. To study the role of conformational changes within the protein catalyst and the DNA substrate, we have determined the structure of the enzyme in the absence of bound DNA, performed gel retardation analyses of DNA binding and bending, and have mutagenized a leucine residue that contacts an adenine nucleotide at the site of cleavage. The structure of the L116A/DNA complex has been determined and the effects of the mutation on affinity and catalysis have been measured. The wild-type protein displays a rigid-body rotation of its individual subunits upon DNA binding. Homing site DNA is not detectably bent in the absence of protein, but is sharply bent in both the wild-type and L116A complexes. These results indicate that binding involves a large distortion of the DNA and a smaller change in protein conformation. Leucine 116 is critical for binding and catalysis: it appears to be important for forming a well-ordered protein-DNA complex at the cleavage site, for maximal deformation of the DNA, and for desolvation of the nucleotide bases that are partially unstacked in the enzyme complex.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1EVX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVX OCA].
+
-
 
+
-
==Reference==
+
-
Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site., Galburt EA, Chadsey MS, Jurica MS, Chevalier BS, Erho D, Tang W, Monnat RJ Jr, Stoddard BL, J Mol Biol. 2000 Jul 21;300(4):877-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10891275 10891275]
+
[[Category: Physarum polycephalum]]
[[Category: Physarum polycephalum]]
-
[[Category: Single protein]]
+
[[Category: Chevalier BS]]
-
[[Category: Chevalier, B S.]]
+
[[Category: Erho D]]
-
[[Category: Erho, D.]]
+
[[Category: Galburt EA]]
-
[[Category: Galburt, E A.]]
+
[[Category: Jurica MS]]
-
[[Category: Jurica, M S.]]
+
[[Category: Stoddard BL]]
-
[[Category: Stoddard, B L.]]
+
-
[[Category: C-terminal exchanged dimer interface]]
+
-
[[Category: Dna binding b-sheet]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:34:58 2008''
+

Current revision

APO CRYSTAL STRUCTURE OF THE HOMING ENDONUCLEASE, I-PPOI

PDB ID 1evx

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1evx"
Personal tools