1ez3

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[[Image:1ez3.jpg|left|200px]]
 
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==CRYSTAL STRUCTURE OF THE NEURONAL T-SNARE SYNTAXIN-1A==
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The line below this paragraph, containing "STRUCTURE_1ez3", creates the "Structure Box" on the page.
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<StructureSection load='1ez3' size='340' side='right'caption='[[1ez3]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ez3]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EZ3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ez3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ez3 OCA], [https://pdbe.org/1ez3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ez3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ez3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ez3 ProSAT]</span></td></tr>
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{{STRUCTURE_1ez3| PDB=1ez3 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/STX1A_RAT STX1A_RAT] Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/1ez3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ez3 ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF THE NEURONAL T-SNARE SYNTAXIN-1A'''
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==See Also==
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*[[Syntaxin 3D structures|Syntaxin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Intracellular trafficking depends on the docking and fusion of transport vesicles with cellular membranes. Central to docking and fusion is the pairing of SNARE proteins (soluble NSF attachment protein receptors) associated with the vesicle and target membranes (v- and t-SNAREs, respectively). Here, the X-ray structure of an N-terminal conserved domain of the neuronal t-SNARE syntaxin-1A was determined to a resolution of 1.9 A using multiwavelength anomalous diffraction. This X-ray structure, which is in general agreement with an NMR structure of a similar fragment, provides new insight into the interaction surface between the N-terminal domain and the remainder of the protein. In vitro characterization of the intact cytoplasmic domain of syntaxin revealed that it forms dimers, and probably tetramers, at low micromolar concentrations, with concomitant structural changes that can be detected by limited proteolysis. These observations suggest that the promiscuity characteristic of pairing between v-SNAREs and t-SNAREs extends to the formation of homo-oligomeric t-SNARE complexes as well. They also suggest a potential role for the neuronal Sec1 protein (nSec1) in preventing the formation of syntaxin multimers.
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[[Category: Large Structures]]
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==About this Structure==
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1EZ3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZ3 OCA].
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==Reference==
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Structural analysis of the neuronal SNARE protein syntaxin-1A., Lerman JC, Robblee J, Fairman R, Hughson FM, Biochemistry. 2000 Jul 25;39(29):8470-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10913252 10913252]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Fairman R]]
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[[Category: Fairman, R.]]
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[[Category: Hughson FM]]
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[[Category: Hughson, F M.]]
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[[Category: Lerman JC]]
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[[Category: Lerman, J C.]]
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[[Category: Robblee J]]
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[[Category: Robblee, J.]]
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[[Category: Three helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:41:46 2008''
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Current revision

CRYSTAL STRUCTURE OF THE NEURONAL T-SNARE SYNTAXIN-1A

PDB ID 1ez3

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