This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7ohf
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Cryo-EM structure of pyrococcus furiosus apoferritin in nanofluidic channels== | ==Cryo-EM structure of pyrococcus furiosus apoferritin in nanofluidic channels== | ||
| - | <StructureSection load='7ohf' size='340' side='right'caption='[[7ohf]]' scene=''> | + | <StructureSection load='7ohf' size='340' side='right'caption='[[7ohf]], [[Resolution|resolution]] 3.00Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHF FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ohf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_com1 Pyrococcus furiosus com1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OHF FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohf OCA], [https://pdbe.org/7ohf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohf RCSB], [https://www.ebi.ac.uk/pdbsum/7ohf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohf ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PFC_02870 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1185654 Pyrococcus furiosus COM1])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ohf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ohf OCA], [https://pdbe.org/7ohf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ohf RCSB], [https://www.ebi.ac.uk/pdbsum/7ohf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ohf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cryogenic electron microscopy has become an essential tool for structure determination of biological macromolecules. In practice, the difficulty to reliably prepare samples with uniform ice thickness still represents a barrier for routine high-resolution imaging and limits the current throughput of the technique. We show that a nanofluidic sample support with well-defined geometry can be used to prepare cryo-EM specimens with reproducible ice thickness from picoliter sample volumes. The sample solution is contained in electron-transparent nanochannels that provide uniform thickness gradients without further optimisation and eliminate the potentially destructive air-water interface. We demonstrate the possibility to perform high-resolution structure determination with three standard protein specimens. Nanofabricated sample supports bear potential to automate the cryo-EM workflow, and to explore new frontiers for cryo-EM applications such as time-resolved imaging and high-throughput screening. | ||
| + | |||
| + | Nanofluidic chips for cryo-EM structure determination from picoliter sample volumes.,Huber ST, Sarajlic E, Huijink R, Weis F, Evers WH, Jakobi AJ Elife. 2022 Jan 21;11. pii: 72629. doi: 10.7554/eLife.72629. PMID:35060902<ref>PMID:35060902</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ohf" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Evers | + | [[Category: Pyrococcus furiosus com1]] |
| - | [[Category: Huber | + | [[Category: Evers, W H]] |
| - | [[Category: Huijink R]] | + | [[Category: Huber, S T]] |
| - | [[Category: Jakobi | + | [[Category: Huijink, R]] |
| - | [[Category: Sarajlic E]] | + | [[Category: Jakobi, A J]] |
| + | [[Category: Sarajlic, E]] | ||
| + | [[Category: Iron storage]] | ||
| + | [[Category: Metal transport]] | ||
Current revision
Cryo-EM structure of pyrococcus furiosus apoferritin in nanofluidic channels
| |||||||||||
