1l1l

From Proteopedia

(Difference between revisions)

Current revision

CRYSTAL STRUCTURE OF B-12 DEPENDENT (CLASS II) RIBONUCLEOTIDE REDUCTASE

Structural highlights

1l1l is a 4 chain structure with sequence from Lactobacillus leichmannii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.75Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RTPR_LACLE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Ribonucleotide reductases (RNRs) catalyze the conversion of ribonucleotides to deoxyribonucleotides, an essential step in DNA biosynthesis and repair. Here we present the crystal structure of class II (coenzyme B12-dependent) ribonucleoside triphosphate reductase (RTPR) from Lactobacillus leichmannii in the apo enzyme form and in complex with the B12 analog adeninylpentylcobalamin at 1.75 and 2.0 A resolution, respectively. This monomeric, allosterically regulated class II RNR retains all the key structural features associated with the catalytic and regulatory machinery of oligomeric RNRs. Surprisingly, the dimer interface responsible for effector binding in class I RNR is preserved through a single 130-residue insertion in the class II structure. Thus, L. leichmannii RNR is a paradigm for the simplest structural entity capable of ribonucleotide reduction, a reaction linking the RNA and DNA worlds.

The crystal structure of class II ribonucleotide reductase reveals how an allosterically regulated monomer mimics a dimer.,Sintchak MD, Arjara G, Kellogg BA, Stubbe J, Drennan CL Nat Struct Biol. 2002 Apr;9(4):293-300. PMID:11875520^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sintchak MD, Arjara G, Kellogg BA, Stubbe J, Drennan CL. The crystal structure of class II ribonucleotide reductase reveals how an allosterically regulated monomer mimics a dimer. Nat Struct Biol. 2002 Apr;9(4):293-300. PMID:11875520 doi:http://dx.doi.org/10.1038/nsb774

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1l1l"

@@ Line 3: / Line 3: @@
 <StructureSection load='1l1l' size='340' side='right'caption='[[1l1l]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1l1l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_leichmanni_i"_(sic)_henneberg_1903 "bacillus leichmanni i" (sic) henneberg 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L1L FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1l1l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactobacillus_leichmannii Lactobacillus leichmannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L1L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L1L FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonucleoside-triphosphate_reductase Ribonucleoside-triphosphate reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.2 1.17.4.2] </span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l1l OCA], [https://pdbe.org/1l1l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l1l RCSB], [https://www.ebi.ac.uk/pdbsum/1l1l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l1l ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/RTPR_LACLE RTPR_LACLE]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 12: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l1/1l1l_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 33: / Line 35: @@
 __TOC__
 </StructureSection>
+[[Category: Lactobacillus leichmannii]]
 [[Category: Large Structures]]
-[[Category: Ribonucleoside-triphosphate reductase]]
+[[Category: Arjara G]]
-[[Category: Arjara, G]]
+[[Category: Drennan CL]]
-[[Category: Drennan, C L]]
+[[Category: Kellogg BA]]
-[[Category: Kellogg, B A]]
+[[Category: Sintchak MD]]
-[[Category: Sintchak, M D]]
+[[Category: Stubbe J]]
-[[Category: Stubbe, J]]
-[[Category: 10-stranded alpha-beta barrel]]
-[[Category: Central finger loop]]
-[[Category: Oxidoreductase]]

1l1l

From Proteopedia

Current revision

CRYSTAL STRUCTURE OF B-12 DEPENDENT (CLASS II) RIBONUCLEOTIDE REDUCTASE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

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