1f2x

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[[Image:1f2x.jpg|left|200px]]
 
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==STRUCTURE OF THE SINGLE-DOMAIN CAMELID ANTIBODY CAB-CA05==
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The line below this paragraph, containing "STRUCTURE_1f2x", creates the "Structure Box" on the page.
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<StructureSection load='1f2x' size='340' side='right'caption='[[1f2x]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1f2x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F2X FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f2x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f2x OCA], [https://pdbe.org/1f2x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f2x RCSB], [https://www.ebi.ac.uk/pdbsum/1f2x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f2x ProSAT]</span></td></tr>
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{{STRUCTURE_1f2x| PDB=1f2x | SCENE= }}
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f2/1f2x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f2x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Grafting the antigen-binding loops onto a human antibody scaffold is a widely used technique to humanise murine antibodies. The success of this approach depends largely on the observation that the antigen-binding loops adopt only a limited number of canonical structures. Identification of the correct canonical structure is therefore essential. Algorithms that predict the main-chain conformation of the hypervariable loops using only the amino acid sequence often provide this information. Here, we describe new canonical loop conformations for the hypervariable regions H1 and H2 as found in single-domain antibody fragments of dromedaries or llama. Although the occurrence of these new loop conformations was not predicted by the algorithms used, it seems that they could occur in human or mouse antigen-binding loops. Their discovery indicates that the currently used set of canonical structures is incomplete and that the prediction algorithms should be extended to include these new structures.
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'''STRUCTURE OF THE SINGLE-DOMAIN CAMELID ANTIBODY CAB-CA05'''
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Canonical antigen-binding loop structures in immunoglobulins: more structures, more canonical classes?,Decanniere K, Muyldermans S, Wyns L J Mol Biol. 2000 Jun 30;300(1):83-91. PMID:10864500<ref>PMID:10864500</ref>
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==Overview==
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Grafting the antigen-binding loops onto a human antibody scaffold is a widely used technique to humanise murine antibodies. The success of this approach depends largely on the observation that the antigen-binding loops adopt only a limited number of canonical structures. Identification of the correct canonical structure is therefore essential. Algorithms that predict the main-chain conformation of the hypervariable loops using only the amino acid sequence often provide this information. Here, we describe new canonical loop conformations for the hypervariable regions H1 and H2 as found in single-domain antibody fragments of dromedaries or llama. Although the occurrence of these new loop conformations was not predicted by the algorithms used, it seems that they could occur in human or mouse antigen-binding loops. Their discovery indicates that the currently used set of canonical structures is incomplete and that the prediction algorithms should be extended to include these new structures.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2X OCA].
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</div>
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<div class="pdbe-citations 1f2x" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Canonical antigen-binding loop structures in immunoglobulins: more structures, more canonical classes?, Decanniere K, Muyldermans S, Wyns L, J Mol Biol. 2000 Jun 30;300(1):83-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10864500 10864500]
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*[[Antibody 3D structures|Antibody 3D structures]]
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[[Category: Decanniere, K.]]
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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[[Category: Muyldermans, S.]]
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== References ==
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[[Category: Wyns, L.]]
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<references/>
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[[Category: Immunoglobulin fold]]
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__TOC__
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:49:50 2008''
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</StructureSection>
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[[Category: Camelus dromedarius]]
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[[Category: Large Structures]]
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[[Category: Decanniere K]]
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[[Category: Muyldermans S]]
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[[Category: Wyns L]]

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STRUCTURE OF THE SINGLE-DOMAIN CAMELID ANTIBODY CAB-CA05

PDB ID 1f2x

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