Journal:Acta Cryst F:S2053230X21008542
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| - | - | + | <StructureSection load='' size='450' side='right' scene='89/890862/Cv/12' caption=''> |
| + | ===Glycogen phosphorylase revisited: extending the resolution of the R- and T-state structures of the free enzyme and in complex with allosteric activators=== | ||
| + | <big>Demetres D. Leonidas, Spyros E. Zographos, Katerina E. Tsitsanou, Vasiliki T. Skamnaki, George Stravodimos, and Efthimios Kyriakis</big> <ref>doi: 10.1107/S2053230X21008542</ref> | ||
| + | <hr/> | ||
| + | <b>Molecular Tour</b><br> | ||
| + | The crystal structures of the free T- and R-state glycogen phosphorylase (GP) and the R-state GP in complex with allosteric activators IMP and AMP are reported, in improved resolution. GP is a validated pharmaceutical target for the development of antihyperglycaemic agents and the reported structures can have significant impact on structure-based drug design efforts. Comparisons to previously reported structures of lower resolution reveal the detailed conformation of important structural features of the allosteric transition from T- to R-state of GP. | ||
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| + | The R-state rmGPb-AMP from a crystal grown in the presence of AMP: | ||
| + | *<scene name='89/890862/Cv/5'>Each tetramer is colored in different colour</scene> | ||
| + | *<scene name='89/890862/Cv/6'>Each subunit is colored in different colour, structurally equivalent subunits in the two tetramers are shown in the same colour</scene> | ||
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| + | <scene name='89/890862/Cv/15'>The different conformation of the N-terminal at the T and R state structures of GPb</scene>. | ||
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| + | <scene name='89/890862/Cv/16'>Animation of this scene</scene>. | ||
| + | <jmol><jmolButton> | ||
| + | <script>if (_animating); anim pause;set echo bottom left; color echo white; font echo 20 sansserif;echo Animation Paused; else; anim resume; set echo off;endif;</script> | ||
| + | <text>If you want to see previous scene click here before</text> | ||
| + | </jmolButton></jmol> | ||
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| + | '''PDB references:''' rabbit muscle glycogen phosphorylase, R-state, [[3e3l]]; T-state, [[7p7d]]; complex with AMP, R-state, [[3e3n]]; complex with IMP, R-state, [[3e3o]]. | ||
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| + | <b>References</b><br> | ||
| + | <references/> | ||
| + | </StructureSection> | ||
| + | __NOEDITSECTION__ | ||
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