7pgj

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(New page: '''Unreleased structure''' The entry 7pgj is ON HOLD Authors: Dinis, P., MetsaKetela, M. Description: Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-decarboxyl...)
Current revision (07:54, 7 February 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 7pgj is ON HOLD
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==Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-decarboxylate TamK and 10-hydroxylase RdmB, together with a single point mutation F297G==
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<StructureSection load='7pgj' size='340' side='right'caption='[[7pgj]], [[Resolution|resolution]] 2.13&Aring;' scene=''>
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Authors: Dinis, P., MetsaKetela, M.
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== Structural highlights ==
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<table><tr><td colspan='2'>[[7pgj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_peucetius Streptomyces peucetius], [https://en.wikipedia.org/wiki/Streptomyces_purpurascens Streptomyces purpurascens] and [https://en.wikipedia.org/wiki/Streptomyces_tsukubensis_NRRL18488 Streptomyces tsukubensis NRRL18488]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGJ FirstGlance]. <br>
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Description: Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-decarboxylate TamK and 10-hydroxylase RdmB, together with a single point mutation F297G
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.13&#8491;</td></tr>
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[[Category: Unreleased Structures]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZBX:methyl+(1R,2R,4S)-2-ethyl-7-methoxy-2,4,5-tris(oxidanyl)-6,11-bis(oxidanylidene)-3,4-dihydro-1H-tetracene-1-carboxylate'>ZBX</scene></td></tr>
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[[Category: Dinis, P]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgj OCA], [https://pdbe.org/7pgj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgj RCSB], [https://www.ebi.ac.uk/pdbsum/7pgj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgj ProSAT]</span></td></tr>
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[[Category: Metsaketela, M]]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RDMB_STREF RDMB_STREF] Involved in the biosynthesis of the anthracycline aclacinomycin which is an aromatic polyketide antibiotic that exhibits high cytotoxicity and is widely applied in the chemotherapy of a variety of cancers. In vivo and in vitro, RdmB catalyzes the removal of the carboxylic group from the C-10 position of 15-demethoxyaclacinomycin T coupled to hydroxylation at the same C-10 position. It could also catalyze the removal of the carboxylic group at the C-10 position of 15-demethoxy-epsilon-rhodomycin coupled to hydroxylation at the same C-10 position to yield rhodomycin B. The reaction catalyzes by RdmB is intriguing, since the enzyme does not use any of the cofactors usually associated with hydroxylases such as flavins and/or metal ions to activate molecular oxygen.<ref>PMID:11004563</ref> <ref>PMID:15548527</ref> [https://www.uniprot.org/uniprot/I2N5E8_STRT9 I2N5E8_STRT9] [https://www.uniprot.org/uniprot/DNRK_STRPE DNRK_STRPE] Involved in the biosynthesis of the anthracyclines carminomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. In vivo, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the 4-O-position of carminomycin to form daunorubicin. In vitro, it also methylates the anthracyclines rhodomycin D (10-carbomethoxy-13-deoxycarminomycin) and 13-deoxy-carminomycin at the 4-hydroxyl position. It is quite specific with respect to the length of the carbohydrate chain at the C7 position, but it can accept substrates with bulky substituent at C10 position.<ref>PMID:15273252</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptomyces peucetius]]
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[[Category: Streptomyces purpurascens]]
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[[Category: Streptomyces tsukubensis NRRL18488]]
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[[Category: Dinis P]]
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[[Category: MetsaKetela M]]

Current revision

Chimeric carminomycin-4-O-methyltransferase (DnrK) with regions from 10-decarboxylate TamK and 10-hydroxylase RdmB, together with a single point mutation F297G

PDB ID 7pgj

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