7v3j
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==DENV2:F(ab')2-local== | |
| + | <StructureSection load='7v3j' size='340' side='right'caption='[[7v3j]], [[Resolution|resolution]] 4.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7v3j]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2_Thailand/NGS-C/1944 Dengue virus 2 Thailand/NGS-C/1944] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V3J FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.9Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v3j OCA], [https://pdbe.org/7v3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v3j RCSB], [https://www.ebi.ac.uk/pdbsum/7v3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v3j ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The human monoclonal antibody (HmAb) C10 potently cross-neutralizes Zika virus (ZIKV) and dengue virus. Analysis of antibody fragment (Fab) C10 interactions with ZIKV and dengue virus serotype 2 (DENV2) particles by cryoelectron microscopy (cryo-EM) and amide hydrogen/deuterium exchange mass spectrometry (HDXMS) shows that Fab C10 binding decreases overall ZIKV particle dynamics, whereas with DENV2, the same Fab causes increased dynamics. Testing of different Fab C10:DENV2 E protein molar ratios revealed that, at higher Fab ratios, especially at saturated concentrations, the Fab enhanced viral dynamics (detected by HDXMS), and observation under cryo-EM showed increased numbers of distorted particles. Our results suggest that Fab C10 stabilizes ZIKV but that with DENV2 particles, high Fab C10 occupancy promotes E protein dimer conformational changes leading to overall increased particle dynamics and distortion of the viral surface. This is the first instance of a broadly neutralizing antibody eliciting virus-specific increases in whole virus particle dynamics. | ||
| - | + | Human antibody C10 neutralizes by diminishing Zika but enhancing dengue virus dynamics.,Lim XX, Shu B, Zhang S, Tan AWK, Ng TS, Lim XN, Chew VS, Shi J, Screaton GR, Lok SM, Anand GS Cell. 2021 Dec 9;184(25):6067-6080.e13. doi: 10.1016/j.cell.2021.11.009. Epub , 2021 Nov 30. PMID:34852238<ref>PMID:34852238</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7v3j" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Antibody 3D structures|Antibody 3D structures]] |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Dengue virus 2 Thailand/NGS-C/1944]] | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lok SM]] | ||
| + | [[Category: Ng TS]] | ||
| + | [[Category: Shu B]] | ||
| + | [[Category: Victor AK]] | ||
| + | [[Category: Zhang S]] | ||
Current revision
DENV2:F(ab')2-local
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