7v5z
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of heterotetrameric complex of Sa2YoeB-Sa2YefM toxin-antitoxin from Staphylococcus aureus== | |
| + | <StructureSection load='7v5z' size='340' side='right'caption='[[7v5z]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7v5z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7V5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7V5Z FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7v5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7v5z OCA], [https://pdbe.org/7v5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7v5z RCSB], [https://www.ebi.ac.uk/pdbsum/7v5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7v5z ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q2FVF7_STAA8 Q2FVF7_STAA8] Antitoxin component of a type II toxin-antitoxin (TA) system.[RuleBase:RU362080] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Toxin-antitoxin (TA) systems are ubiquitous regulatory modules for bacterial growth and cell survival following stress. YefM-YoeB, the most prevalent type II TA system, is present in a variety of bacterial species. In Staphylococcus aureus, the YefM-YoeB system exists as two independent paralogous copies. Our previous research resolved crystal structures of the two oligomeric states (heterotetramer and heterohexamer-DNA ternary complex) of the first paralog as well as the molecular mechanism of transcriptional autoregulation of this module. However, structural details reflecting molecular diversity in both paralogs have been relatively unexplored. To understand the molecular mechanism of how Sa(2)YoeB and Sa(2)YefM regulate their own transcription and how each paralog functions independently, we solved a series of crystal structures of the Sa(2)YoeB-Sa(2)YefM. Our structural and biochemical data demonstrated that both paralogous copies adopt similar mechanisms of transcriptional autoregulation. In addition, structural analysis suggested that molecular diversity between the two paralogs might be reflected in the interaction profile of YefM and YoeB and the recognition pattern of promoter DNA by YefM. Interaction analysis revealed unique conformational and activating force effected by the interface between Sa(2)YoeB and Sa(2)YefM. In addition, the recognition pattern analysis demonstrated that residues Thr(7) and Tyr(14) of Sa(2)YefM specifically recognizes the flanking sequences (G and C) of the promoter DNA. Together, these results provide the structural insights into the molecular diversity and independent function of the paralogous copies of the YoeB-YefM TA system. | ||
| - | + | The two paralogous copies of the YoeB-YefM toxin-antitoxin module in Staphylococcus aureus differ in DNA binding and recognition patterns.,Xue L, Khan MH, Yue J, Zhu Z, Niu L J Biol Chem. 2022 Jan;298(1):101457. doi: 10.1016/j.jbc.2021.101457. Epub 2021 , Nov 30. PMID:34861238<ref>PMID:34861238</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7v5z" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Staphylococcus aureus subsp. aureus NCTC 8325]] | ||
| + | [[Category: Khan MH]] | ||
| + | [[Category: Xue L]] | ||
| + | [[Category: Yue J]] | ||
Current revision
Crystal structure of heterotetrameric complex of Sa2YoeB-Sa2YefM toxin-antitoxin from Staphylococcus aureus
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