1nm3

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of Heamophilus influenza hybrid-Prx5

Structural highlights

1nm3 is a 2 chain structure with sequence from Haemophilus influenzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cellular redox control is often mediated by oxidation and reduction of cysteine residues in the redox-sensitive proteins, where thioredoxin and glutaredoxin (Grx) play as electron donors for the oxidized proteins. Despite the importance of protein-protein interactions between the electron donor and acceptor proteins, there has been no structural information for the interaction of thioredoxin or Grx with natural target proteins. Here, we present the crystal structure of a novel Haemophilus influenza peroxiredoxin (Prx) hybrid Prx5 determined at 2.8-A resolution. The structure reveals that hybrid Prx5 forms a tightly associated tetramer where active sites of Prx and Grx domains of different monomers interact with each other. The Prx-Grx interface comprises specific charge interactions surrounded by weak interactions, providing insight into the target recognition mechanism of Grx. The tetrameric structure also exhibits a flexible active site and alternative Prx-Grx interactions, which appear to facilitate the electron transfer from Grx to Prx domain. Differences of electron donor binding surfaces in Prx proteins revealed by an analysis based on the structural information explain the electron donor specificities of various Prx proteins.

The tetrameric structure of Haemophilus influenza hybrid Prx5 reveals interactions between electron donor and acceptor proteins.,Kim SJ, Woo JR, Hwang YS, Jeong DG, Shin DH, Kim K, Ryu SE J Biol Chem. 2003 Mar 21;278(12):10790-8. Epub 2003 Jan 14. PMID:12529327^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kim SJ, Woo JR, Hwang YS, Jeong DG, Shin DH, Kim K, Ryu SE. The tetrameric structure of Haemophilus influenza hybrid Prx5 reveals interactions between electron donor and acceptor proteins. J Biol Chem. 2003 Mar 21;278(12):10790-8. Epub 2003 Jan 14. PMID:12529327 doi:http://dx.doi.org/10.1074/jbc.M209553200

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nm3"

@@ Line 3: / Line 3: @@
 <StructureSection load='1nm3' size='340' side='right'caption='[[1nm3]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1nm3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NM3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NM3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1nm3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NM3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NM3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nm3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nm3 OCA], [https://pdbe.org/1nm3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nm3 RCSB], [https://www.ebi.ac.uk/pdbsum/1nm3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nm3 ProSAT]</span></td></tr>
 </table>
@@ Line 13: / Line 13: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nm/1nm3_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 31: / Line 31: @@
 __TOC__
 </StructureSection>
-[[Category: Bacterium influenzae lehmann and neumann 1896]]
+[[Category: Haemophilus influenzae]]
 [[Category: Large Structures]]
-[[Category: Hwang, Y S]]
+[[Category: Hwang YS]]
-[[Category: Jeong, D G]]
+[[Category: Jeong DG]]
-[[Category: Kim, K H]]
+[[Category: Kim KH]]
-[[Category: Kim, S J]]
+[[Category: Kim SJ]]
-[[Category: Ryu, S E]]
+[[Category: Ryu SE]]
-[[Category: Shin, D H]]
+[[Category: Shin DH]]
-[[Category: Woo, J R]]
+[[Category: Woo JR]]
-[[Category: Electron transport]]
-[[Category: Glutaredoxin]]
-[[Category: Hybrid]]
-[[Category: Peroxiredoxin]]

1nm3

From Proteopedia

Current revision

Crystal structure of Heamophilus influenza hybrid-Prx5

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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