7apd
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Bovine Papillomavirus E1 DNA helicase-replication fork complex== | |
| + | <StructureSection load='7apd' size='340' side='right'caption='[[7apd]], [[Resolution|resolution]] 3.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7apd]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_papillomavirus Bovine papillomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7APD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7APD FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.9Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7apd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7apd OCA], [https://pdbe.org/7apd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7apd RCSB], [https://www.ebi.ac.uk/pdbsum/7apd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7apd ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/VE1_BPV1 VE1_BPV1] ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hexameric helicases are motor proteins that unwind double-stranded DNA (dsDNA) during DNA replication but how they are optimised for strand separation is unclear. Here we present the cryo-EM structure of the full-length E1 helicase from papillomavirus, revealing all arms of a bound DNA replication fork and their interactions with the helicase. The replication fork junction is located at the entrance to the helicase collar ring, that sits above the AAA + motor assembly. dsDNA is escorted to and the 5 single-stranded DNA (ssDNA) away from the unwinding point by the E1 dsDNA origin binding domains. The 3 ssDNA interacts with six spirally-arranged beta-hairpins and their cyclical top-to-bottom movement pulls the ssDNA through the helicase. Pulling of the RF against the collar ring separates the base-pairs, while modelling of the conformational cycle suggest an accompanying movement of the collar ring has an auxiliary role, helping to make efficient use of ATP in duplex unwinding. | ||
| - | + | Unwinding of a DNA replication fork by a hexameric viral helicase.,Javed A, Major B, Stead JA, Sanders CM, Orlova EV Nat Commun. 2021 Sep 20;12(1):5535. doi: 10.1038/s41467-021-25843-6. PMID:34545080<ref>PMID:34545080</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7apd" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Helicase 3D structures|Helicase 3D structures]] |
| - | [[Category: | + | *[[Replication protein E1|Replication protein E1]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bovine papillomavirus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Javed A]] | ||
| + | [[Category: Major B]] | ||
| + | [[Category: Orlova EV]] | ||
| + | [[Category: Sanders CM]] | ||
| + | [[Category: Stead J]] | ||
Current revision
Bovine Papillomavirus E1 DNA helicase-replication fork complex
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