7rwl
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Envelope-associated Adeno-associated virus serotype 2== | |
| + | <StructureSection load='7rwl' size='340' side='right'caption='[[7rwl]], [[Resolution|resolution]] 3.14Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RWL FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.14Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rwl OCA], [https://pdbe.org/7rwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rwl RCSB], [https://www.ebi.ac.uk/pdbsum/7rwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rwl ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Adeno-associated virus (AAV) are classified as non-enveloped ssDNA viruses. However, AAV capsids embedded within exosomes have been observed, and it has been suggested that the AAV membrane associated accessory protein (MAAP) may play a role in envelope-associated AAV (EA-AAV) capsid formation. Here, we observed and selected sufficient homogeneous EA-AAV capsids of AAV2, produced using the Sf9 baculoviral expression system, to determine the cryo-electron microscopy (cryo-EM) structure at 3.14 A resolution. The reconstructed map confirmed that the EA-AAV capsid, showed no significant structural variation compared to the non-envelope capsid. In addition, the Sf9 expression system used implies the notion that MAAP may enhance exosome AAV encapsulation. Furthermore, we speculate that these EA-AAV capsids may have therapeutic benefits over the currently used non-envelope AAV capsids, with advantages in immune evasion and/or improved infectivity. | ||
| - | + | Structural characterization of an envelope-associated adeno-associated virus type 2 capsid.,Hull JA, Mietzsch M, Chipman P, Strugatsky D, McKenna R Virology. 2021 Oct 6;565:22-28. doi: 10.1016/j.virol.2021.09.010. PMID:34638006<ref>PMID:34638006</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7rwl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chipman P]] | ||
| + | [[Category: Hull JA]] | ||
| + | [[Category: McKenna R]] | ||
| + | [[Category: Mietzsch M]] | ||
| + | [[Category: Strugatsky D]] | ||
Current revision
Envelope-associated Adeno-associated virus serotype 2
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