1f5u

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[[Image:1f5u.gif|left|200px]]
 
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==SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA==
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The line below this paragraph, containing "STRUCTURE_1f5u", creates the "Structure Box" on the page.
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<StructureSection load='1f5u' size='340' side='right'caption='[[1f5u]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1f5u]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F5U FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f5u OCA], [https://pdbe.org/1f5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f5u RCSB], [https://www.ebi.ac.uk/pdbsum/1f5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f5u ProSAT]</span></td></tr>
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{{STRUCTURE_1f5u| PDB=1f5u | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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'''SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA'''
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== Publication Abstract from PubMed ==
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==Overview==
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The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs.
The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs.
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==About this Structure==
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A retroviral RNA kissing complex containing only two G.C base pairs.,Kim CH, Tinoco I Jr Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:10931958<ref>PMID:10931958</ref>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5U OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A retroviral RNA kissing complex containing only two G.C base pairs., Kim CH, Tinoco I Jr, Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10931958 10931958]
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</div>
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[[Category: Jr., I Tinoco.]]
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<div class="pdbe-citations 1f5u" style="background-color:#fffaf0;"></div>
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[[Category: Kim, C H.]]
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== References ==
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[[Category: Dimerization]]
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<references/>
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[[Category: Kissing dimer]]
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__TOC__
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[[Category: Moloney murine leukemia virus]]
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</StructureSection>
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[[Category: Nmr]]
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[[Category: Large Structures]]
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[[Category: Retrovirus]]
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[[Category: Kim C-H]]
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[[Category: Stem-loop]]
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[[Category: Tinoco Jr I]]
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[[Category: Tetraloop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:56:14 2008''
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Current revision

SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA

PDB ID 1f5u

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