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| | <StructureSection load='6wpz' size='340' side='right'caption='[[6wpz]], [[Resolution|resolution]] 1.99Å' scene=''> | | <StructureSection load='6wpz' size='340' side='right'caption='[[6wpz]], [[Resolution|resolution]] 1.99Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6wpz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WPZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6wpz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WPZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.993Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CGU42_23305 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpz OCA], [https://pdbe.org/6wpz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wpz RCSB], [https://www.ebi.ac.uk/pdbsum/6wpz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpz ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpz OCA], [https://pdbe.org/6wpz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wpz RCSB], [https://www.ebi.ac.uk/pdbsum/6wpz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A509JD33_PSEAI A0A509JD33_PSEAI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Duggin, I G]] | + | [[Category: Pseudomonas aeruginosa]] |
| - | [[Category: McDougald, D]] | + | [[Category: Duggin IG]] |
| - | [[Category: Michie, K A]] | + | [[Category: McDougald D]] |
| - | [[Category: Norrian, P]] | + | [[Category: Michie KA]] |
| - | [[Category: Rice, S A]] | + | [[Category: Norrian P]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Rice SA]] |
| Structural highlights
Function
A0A509JD33_PSEAI
Publication Abstract from PubMed
It has been shown that the filamentous phage, Pf4, plays an important role in biofilm development, stress tolerance, genetic variant formation and virulence in Pseudomonas aeruginosa PAO1. These behaviours are linked to the appearance of superinfective phage variants. Here, we have investigated the molecular mechanism of superinfection as well as how the Pf4 phage can control host gene expression to modulate host behaviours. Pf4 exists as a prophage in PAO1 and encodes a homologue of the P2 phage repressor C and was recently named Pf4r. Through a combination of molecular techniques, ChIPseq and transcriptomic analyses, we show a critical site in repressor C (Pf4r) where a mutation in the site, 788799A>G (Ser4Pro), causes Pf4r to lose its function as the immunity factor against reinfection by Pf4. X-ray crystal structure analysis shows that Pf4r forms symmetric homo-dimers homologous to the E.coli bacteriophage P2 RepC protein. A mutation, Pf4r*, associated with the superinfective Pf4r variant, found at the dimer interface, suggests dimer formation may be disrupted, which derepresses phage replication. This is supported by multi-angle light scattering (MALS) analysis, where the Pf4r* protein only forms monomers. The loss of dimerisation also explains the loss of Pf4r's immunity function. Phenotypic assays showed that Pf4r increased LasB activity and was also associated with a slight increase in the percentage of morphotypic variants. ChIPseq and transcriptomic analyses suggest that Pf4r also likely functions as a transcriptional regulator for other host genes. Collectively, these data suggest the mechanism by which filamentous phages play such an important role in P. aeruginosa biofilm development.
The Repressor C Protein, Pf4r, Controls Superinfection of Pseudomonas aeruginosa PAO1 by the Pf4 Filamentous Phage and Regulates Host Gene Expression.,Ismail MH, Michie KA, Goh YF, Noorian P, Kjelleberg S, Duggin IG, McDougald D, Rice SA Viruses. 2021 Aug 15;13(8). pii: v13081614. doi: 10.3390/v13081614. PMID:34452479[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ismail MH, Michie KA, Goh YF, Noorian P, Kjelleberg S, Duggin IG, McDougald D, Rice SA. The Repressor C Protein, Pf4r, Controls Superinfection of Pseudomonas aeruginosa PAO1 by the Pf4 Filamentous Phage and Regulates Host Gene Expression. Viruses. 2021 Aug 15;13(8). pii: v13081614. doi: 10.3390/v13081614. PMID:34452479 doi:http://dx.doi.org/10.3390/v13081614
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