7me1

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<StructureSection load='7me1' size='340' side='right'caption='[[7me1]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
<StructureSection load='7me1' size='340' side='right'caption='[[7me1]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[7me1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ME1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ME1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7me1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ME1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ME1 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yfeA, YPO2439, y1897, YP_2227 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7me1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7me1 OCA], [https://pdbe.org/7me1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7me1 RCSB], [https://www.ebi.ac.uk/pdbsum/7me1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7me1 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7me1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7me1 OCA], [https://pdbe.org/7me1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7me1 RCSB], [https://www.ebi.ac.uk/pdbsum/7me1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7me1 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/YFEA_YERPE YFEA_YERPE]] Part of an ATP-driven transport system YfeABCD for chelated iron.
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[https://www.uniprot.org/uniprot/YFEA_YERPE YFEA_YERPE] Part of an ATP-driven transport system YfeABCD for chelated iron.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The substrate-binding protein YfeA (also known as YPO2439 or y1897) is a polyspecific metal-binding protein that is crucial for nutrient acquisition and virulence in Yersinia pestis, the causative microbe of plague. YfeA folds into a monomeric c-clamp like other substrate-binding proteins and has two metal-binding sites (sites 1 and 2). Site 2 is a bidentate surface site capable of binding Zn and Mn atoms and is a unique feature of YfeA. Occasionally, the site 2 residues of two YfeA molecules will cooperate with the histidine tag of a third YfeA molecule in coordinating the same metal and lead to metal-dependent crystallographic packing. Here, three crystal structures of YfeA are presented at 1.85, 2.05 and 2.25 A resolution. A comparison of the structures reveals that the metal can be displaced at five different locations ranging from approximately 4 to approximately 16 A away from the canonical site 2. These observations reveal different configurations of site 2 that enable cooperative metal binding and demonstrate how site 2 is dynamic and freely available for inter-protein metal coordination.
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Site 2 of the Yersinia pestis substrate-binding protein YfeA is a dynamic surface metal-binding site.,Radka CD, Aller SG Acta Crystallogr F Struct Biol Commun. 2021 Sep 1;77(Pt 9):286-293. doi:, 10.1107/S2053230X21008086. Epub 2021 Aug 24. PMID:34473105<ref>PMID:34473105</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7me1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Aller, S G]]
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[[Category: Yersinia pestis]]
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[[Category: Radka, C D]]
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[[Category: Aller SG]]
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[[Category: Metal coordination]]
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[[Category: Radka CD]]
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[[Category: Metal transport]]
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Current revision

YfeA oligomer crystal 1, form 1

PDB ID 7me1

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