1f6b

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[[Image:1f6b.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF SAR1-GDP COMPLEX==
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The line below this paragraph, containing "STRUCTURE_1f6b", creates the "Structure Box" on the page.
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<StructureSection load='1f6b' size='340' side='right'caption='[[1f6b]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1f6b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F6B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1f6b| PDB=1f6b | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f6b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f6b OCA], [https://pdbe.org/1f6b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f6b RCSB], [https://www.ebi.ac.uk/pdbsum/1f6b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f6b ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF SAR1-GDP COMPLEX'''
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== Function ==
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[https://www.uniprot.org/uniprot/SAR1B_CRIGR SAR1B_CRIGR] Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.<ref>PMID:8138575</ref> <ref>PMID:11149932</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The Sar1 GTPase is an essential component of COPII vesicle coats involved in export of cargo from the ER. We report the 1.7-A structure of Sar1 and find that consistent with the sequence divergence of Sar1 from Arf family GTPases, Sar1 is structurally distinct. In particular, we show that the Sar1 NH2 terminus contains two regions: an NH2-terminal extension containing an evolutionary conserved hydrophobic motif that facilitates membrane recruitment and activation by the mammalian Sec12 guanine nucleotide exchange factor, and an alpha1' amphipathic helix that contributes to interaction with the Sec23/24 complex that is responsible for cargo selection during ER export. We propose that the hydrophobic Sar1 NH2-terminal activation/recruitment motif, in conjunction with the alpha1' helix, mediates the initial steps in COPII coat assembly for export from the ER.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f6/1f6b_consurf.spt"</scriptWhenChecked>
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1F6B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F6B OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of Sar1-GDP at 1.7 A resolution and the role of the NH2 terminus in ER export., Huang M, Weissman JT, Beraud-Dufour S, Luan P, Wang C, Chen W, Aridor M, Wilson IA, Balch WE, J Cell Biol. 2001 Dec 10;155(6):937-48. Epub 2001 Dec 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11739406 11739406]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f6b ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Cricetulus griseus]]
[[Category: Cricetulus griseus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Balch, W E.]]
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[[Category: Balch WE]]
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[[Category: Huang, M.]]
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[[Category: Huang M]]
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[[Category: Wilson, I A.]]
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[[Category: Wilson IA]]
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[[Category: Gtpase]]
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[[Category: Mg-containing complex]]
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[[Category: N-terminal helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:57:22 2008''
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Current revision

CRYSTAL STRUCTURE OF SAR1-GDP COMPLEX

PDB ID 1f6b

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