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1bw6

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(New page: 200px<br /> <applet load="1bw6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bw6" /> '''HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BIN...)
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[[Image:1bw6.gif|left|200px]]<br />
 
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<applet load="1bw6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bw6" />
 
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'''HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1'''<br />
 
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==Overview==
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==HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1==
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CENP-B has been suggested to organize arrays of centromere satellite DNA, into a higher order structure which then directs centromere formation and, kinetochore assembly in mammalian chromosomes. The N-terminal portion of, CENP-B is a 15 kDa DNA binding domain (DBD) consisting of two repeating, units, RP1 and RP2. The DBD specifically binds to the CENP-B box sequence, (17 bp) in centromere DNA. We determined the solution structure of human, CENP-B DBD RP1 by multi-dimensional 1H, 13C and 15N NMR methods. The, CENP-B DBD RP1 structure consists of four helices and has a, helix-turn-helix structure. The overall folding is similar to those of, some other eukaryotic DBDs, although significant sequence homology with, these proteins was not found. The DBD of yeast RAP1, a telomere binding, protein, is most similar to CENP-B DBD RP1. We studied the interaction, between CENP-B DBD RP1 and the CENP-B box by the use of NMR chemical shift, perturbation. The results suggest that CENP-B DBD RP1 interacts with one, of the essential regions of the CENP-B box DNA, mainly at the N-terminal, basic region, the N-terminal portion of helix 2 and helix 3.
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<StructureSection load='1bw6' size='340' side='right'caption='[[1bw6]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bw6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BW6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BW6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bw6 OCA], [https://pdbe.org/1bw6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bw6 RCSB], [https://www.ebi.ac.uk/pdbsum/1bw6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bw6 ProSAT], [https://www.topsan.org/Proteins/RSGI/1bw6 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CENPB_HUMAN CENPB_HUMAN] Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CENP-B has been suggested to organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. The N-terminal portion of CENP-B is a 15 kDa DNA binding domain (DBD) consisting of two repeating units, RP1 and RP2. The DBD specifically binds to the CENP-B box sequence (17 bp) in centromere DNA. We determined the solution structure of human CENP-B DBD RP1 by multi-dimensional 1H, 13C and 15N NMR methods. The CENP-B DBD RP1 structure consists of four helices and has a helix-turn-helix structure. The overall folding is similar to those of some other eukaryotic DBDs, although significant sequence homology with these proteins was not found. The DBD of yeast RAP1, a telomere binding protein, is most similar to CENP-B DBD RP1. We studied the interaction between CENP-B DBD RP1 and the CENP-B box by the use of NMR chemical shift perturbation. The results suggest that CENP-B DBD RP1 interacts with one of the essential regions of the CENP-B box DNA, mainly at the N-terminal basic region, the N-terminal portion of helix 2 and helix 3.
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==About this Structure==
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A helix-turn-helix structure unit in human centromere protein B (CENP-B).,Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S EMBO J. 1998 Feb 2;17(3):827-37. PMID:9451007<ref>PMID:9451007</ref>
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1BW6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BW6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A helix-turn-helix structure unit in human centromere protein B (CENP-B)., Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S, EMBO J. 1998 Feb 2;17(3):827-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9451007 9451007]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1bw6" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Iwahara, J.]]
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[[Category: Kigawa, T.]]
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[[Category: Kitagawa, K.]]
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[[Category: Masumoto, H.]]
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[[Category: Okazaki, T.]]
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[[Category: RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.]]
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[[Category: Yokoyama, S.]]
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[[Category: centromere protein]]
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[[Category: dna-binding]]
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[[Category: helix-turn-helix]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: rsgi]]
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[[Category: structural genomics]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:14:57 2007''
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==See Also==
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*[[Centromere protein 3D structure|Centromere protein 3D structure]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Iwahara J]]
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[[Category: Kigawa T]]
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[[Category: Kitagawa K]]
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[[Category: Masumoto H]]
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[[Category: Okazaki T]]
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[[Category: Yokoyama S]]

Current revision

HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1

PDB ID 1bw6

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