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| - | <!-- | + | ==THE 0.97 RESOLUTION STRUCTURE OF BUCANDIN, A NOVEL TOXIN ISOLATED FROM THE MALAYAN KRAIT== |
| - | The line below this paragraph, containing "STRUCTURE_1f94", creates the "Structure Box" on the page.
| + | <StructureSection load='1f94' size='340' side='right'caption='[[1f94]], [[Resolution|resolution]] 0.97Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1f94]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_candidus Bungarus candidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F94 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97Å</td></tr> |
| - | --> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f94 OCA], [https://pdbe.org/1f94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f94 RCSB], [https://www.ebi.ac.uk/pdbsum/1f94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f94 ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1f94| PDB=1f94 | SCENE= }}
| + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/3NOJ_BUNCA 3NOJ_BUNCA] This toxin is described as enhancing presynaptic acetylcholine release, but neither experimental results, nor references to other sources are available.<ref>PMID:11053837</ref> |
| | + | <div style="background-color:#fffaf0;"> |
| | + | == Publication Abstract from PubMed == |
| | + | Bucandin is a novel presynaptic neurotoxin isolated from Bungarus candidus (Malayan krait). It has the unique property of enhancing presynaptic acetylcholine release and represents a family of three-finger toxins with an additional disulfide in the first loop. There are no existing structures from this sub-category of three-finger toxins. The X-ray crystal structure of bucandin has been determined by the Shake-and-Bake direct-methods procedure. The resulting electron-density maps were of outstanding quality and allowed the automated tracing of 61 of the 63 amino-acid residues, including their side chains, and the placement of 48 solvent molecules. The 0.97 A resolution full-matrix least-squares refinement converged to a crystallographic R factor of 12.4% and the final model contains 118 solvent molecules. This is the highest resolution structure of any member of the three-finger toxin family and thus it can serve as the best model for other members of the family. Furthermore, the structure of this novel toxin will help in understanding its unique ability to enhance acetylcholine release. The unique structure resulting from the fifth disulfide bond residing in the first loop improves the understanding of other toxins with a similar arrangement of disulfide bonds. |
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| - | '''THE 0.97 RESOLUTION STRUCTURE OF BUCANDIN, A NOVEL TOXIN ISOLATED FROM THE MALAYAN KRAIT'''
| + | The atomic resolution structure of bucandin, a novel toxin isolated from the Malayan krait, determined by direct methods.,Kuhn P, Deacon AM, Comoso S, Rajaseger G, Kini RM, Uson I, Kolatkar PR Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1401-7. PMID:11053837<ref>PMID:11053837</ref> |
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| - | | + | |
| - | ==Overview==
| + | |
| - | Bucandin is a novel presynaptic neurotoxin isolated from Bungarus candidus (Malayan krait). It has the unique property of enhancing presynaptic acetylcholine release and represents a family of three-finger toxins with an additional disulfide in the first loop. There are no existing structures from this sub-category of three-finger toxins. The X-ray crystal structure of bucandin has been determined by the Shake-and-Bake direct-methods procedure. The resulting electron-density maps were of outstanding quality and allowed the automated tracing of 61 of the 63 amino-acid residues, including their side chains, and the placement of 48 solvent molecules. The 0.97 A resolution full-matrix least-squares refinement converged to a crystallographic R factor of 12.4% and the final model contains 118 solvent molecules. This is the highest resolution structure of any member of the three-finger toxin family and thus it can serve as the best model for other members of the family. Furthermore, the structure of this novel toxin will help in understanding its unique ability to enhance acetylcholine release. The unique structure resulting from the fifth disulfide bond residing in the first loop improves the understanding of other toxins with a similar arrangement of disulfide bonds.
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| | | | |
| - | ==About this Structure==
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | 1F94 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bungarus_candidus Bungarus candidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F94 OCA].
| + | </div> |
| | + | <div class="pdbe-citations 1f94" style="background-color:#fffaf0;"></div> |
| | | | |
| - | ==Reference== | + | ==See Also== |
| - | The atomic resolution structure of bucandin, a novel toxin isolated from the Malayan krait, determined by direct methods., Kuhn P, Deacon AM, Comoso S, Rajaseger G, Kini RM, Uson I, Kolatkar PR, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1401-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11053837 11053837]
| + | *[[Bucandin|Bucandin]] |
| | + | == References == |
| | + | <references/> |
| | + | __TOC__ |
| | + | </StructureSection> |
| | [[Category: Bungarus candidus]] | | [[Category: Bungarus candidus]] |
| - | [[Category: Single protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Comoso, S.]] | + | [[Category: Comoso S]] |
| - | [[Category: Deacon, A M.]] | + | [[Category: Deacon AM]] |
| - | [[Category: Kini, R M.]] | + | [[Category: Kini RM]] |
| - | [[Category: Kolatkar, P R.]] | + | [[Category: Kolatkar PR]] |
| - | [[Category: Kuhn, P.]] | + | [[Category: Kuhn P]] |
| - | [[Category: Rajaseger, G.]] | + | [[Category: Rajaseger G]] |
| - | [[Category: Uson, I.]] | + | [[Category: Uson I]] |
| - | [[Category: Three-finger snake presynaptic neurotoxin]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:03:06 2008''
| + | |
| Structural highlights
Function
3NOJ_BUNCA This toxin is described as enhancing presynaptic acetylcholine release, but neither experimental results, nor references to other sources are available.[1]
Publication Abstract from PubMed
Bucandin is a novel presynaptic neurotoxin isolated from Bungarus candidus (Malayan krait). It has the unique property of enhancing presynaptic acetylcholine release and represents a family of three-finger toxins with an additional disulfide in the first loop. There are no existing structures from this sub-category of three-finger toxins. The X-ray crystal structure of bucandin has been determined by the Shake-and-Bake direct-methods procedure. The resulting electron-density maps were of outstanding quality and allowed the automated tracing of 61 of the 63 amino-acid residues, including their side chains, and the placement of 48 solvent molecules. The 0.97 A resolution full-matrix least-squares refinement converged to a crystallographic R factor of 12.4% and the final model contains 118 solvent molecules. This is the highest resolution structure of any member of the three-finger toxin family and thus it can serve as the best model for other members of the family. Furthermore, the structure of this novel toxin will help in understanding its unique ability to enhance acetylcholine release. The unique structure resulting from the fifth disulfide bond residing in the first loop improves the understanding of other toxins with a similar arrangement of disulfide bonds.
The atomic resolution structure of bucandin, a novel toxin isolated from the Malayan krait, determined by direct methods.,Kuhn P, Deacon AM, Comoso S, Rajaseger G, Kini RM, Uson I, Kolatkar PR Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1401-7. PMID:11053837[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kuhn P, Deacon AM, Comoso S, Rajaseger G, Kini RM, Uson I, Kolatkar PR. The atomic resolution structure of bucandin, a novel toxin isolated from the Malayan krait, determined by direct methods. Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1401-7. PMID:11053837
- ↑ Kuhn P, Deacon AM, Comoso S, Rajaseger G, Kini RM, Uson I, Kolatkar PR. The atomic resolution structure of bucandin, a novel toxin isolated from the Malayan krait, determined by direct methods. Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1401-7. PMID:11053837
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