1c25

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:40, 7 February 2024) (edit) (undo)
 
(18 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1c25.gif|left|200px]]<br />
 
-
<applet load="1c25" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1c25, resolution 2.3&Aring;" />
 
-
'''HUMAN CDC25A CATALYTIC DOMAIN'''<br />
 
-
==Overview==
+
==HUMAN CDC25A CATALYTIC DOMAIN==
-
Cdc25 phosphatases activate the cell division kinases throughout the cell, cycle. The 2.3 A structure of the human Cdc25A catalytic domain reveals a, small alpha/beta domain with a fold unlike previously described, phosphatase structures but identical to rhodanese, a sulfur-transfer, protein. Only the active-site loop, containing the Cys-(X)5-Arg motif, shows similarity to the tyrosine phosphatases. In some crystals, the, catalytic Cys-430 forms a disulfide bond with the invariant Cys-384, suggesting that Cdc25 may be self-inhibited during oxidative stress., Asp-383, previously proposed to be the general acid, instead serves a, structural role, forming a conserved buried salt-bridge. We propose that, Glu-431 may act as a general acid. Structure-based alignments suggest that, the noncatalytic domain of the MAP kinase phosphatases will share this, topology, as will ACR2, a eukaryotic arsenical resistance protein.
+
<StructureSection load='1c25' size='340' side='right'caption='[[1c25]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1c25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C25 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c25 OCA], [https://pdbe.org/1c25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c25 RCSB], [https://www.ebi.ac.uk/pdbsum/1c25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c25 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MPIP1_HUMAN MPIP1_HUMAN] Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c2/1c25_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c25 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1C25 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Structure known Active Sites: DSU and POP. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C25 OCA].
+
*[[Tyrosine phosphatase 3D structures|Tyrosine phosphatase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure of the catalytic domain of the human cell cycle control phosphatase, Cdc25A., Fauman EB, Cogswell JP, Lovejoy B, Rocque WJ, Holmes W, Montana VG, Piwnica-Worms H, Rink MJ, Saper MA, Cell. 1998 May 15;93(4):617-25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9604936 9604936]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Protein-tyrosine-phosphatase]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Cogswell JP]]
-
[[Category: Cogswell, J.P.]]
+
[[Category: Fauman EB]]
-
[[Category: Fauman, E.B.]]
+
[[Category: Holmes W]]
-
[[Category: Holmes, W.]]
+
[[Category: Lovejoy B]]
-
[[Category: Lovejoy, B.]]
+
[[Category: Montana VG]]
-
[[Category: Montana, V.G.]]
+
[[Category: Piwnica-Worms H]]
-
[[Category: Piwnica-Worms, H.]]
+
[[Category: Rink MJ]]
-
[[Category: Rink, M.J.]]
+
[[Category: Rocque WJ]]
-
[[Category: Rocque, W.J.]]
+
[[Category: Saper MA]]
-
[[Category: Saper, M.A.]]
+
-
[[Category: cdk2]]
+
-
[[Category: cell cycle phosphatase]]
+
-
[[Category: dual specificity protein phosphatase]]
+
-
[[Category: hydrolase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:16:52 2007''
+

Current revision

HUMAN CDC25A CATALYTIC DOMAIN

PDB ID 1c25

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1c25"
Personal tools