7nli
From Proteopedia
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==S. cerevisiae Ty1 p22 restriction factor, Gag CA-CTD, AUG2 variant== | ==S. cerevisiae Ty1 p22 restriction factor, Gag CA-CTD, AUG2 variant== | ||
| - | <StructureSection load='7nli' size='340' side='right'caption='[[7nli]]' scene=''> | + | <StructureSection load='7nli' size='340' side='right'caption='[[7nli]], [[Resolution|resolution]] 3.12Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NLI FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NLI FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nli OCA], [https://pdbe.org/7nli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nli RCSB], [https://www.ebi.ac.uk/pdbsum/7nli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nli ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.119Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nli OCA], [https://pdbe.org/7nli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nli RCSB], [https://www.ebi.ac.uk/pdbsum/7nli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nli ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Excessive replication of Saccharomyces cerevisiae Ty1 retrotransposons is regulated by Copy Number Control, a process requiring the p22/p18 protein produced from a sub-genomic transcript initiated within Ty1 GAG. In retrotransposition, Gag performs the capsid functions required for replication and re-integration. To minimize genomic damage, p22/p18 interrupts virus-like particle function by interaction with Gag. Here, we present structural, biophysical and genetic analyses of p18m, a minimal fragment of Gag that restricts transposition. The 2.8 A crystal structure of p18m reveals an all alpha-helical protein related to mammalian and insect ARC proteins. p18m retains the capacity to dimerise in solution and the crystal structures reveal two exclusive dimer interfaces. We probe our findings through biophysical analysis of interface mutants as well as Ty1 transposition and p18m restriction in vivo. Our data provide insight into Ty1 Gag structure and suggest how p22/p18 might function in restriction through a blocking-of-assembly mechanism. | ||
| + | |||
| + | Structure of a Ty1 restriction factor reveals the molecular basis of transposition copy number control.,Cottee MA, Beckwith SL, Letham SC, Kim SJ, Young GR, Stoye JP, Garfinkel DJ, Taylor IA Nat Commun. 2021 Sep 22;12(1):5590. doi: 10.1038/s41467-021-25849-0. PMID:34552077<ref>PMID:34552077</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7nli" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
S. cerevisiae Ty1 p22 restriction factor, Gag CA-CTD, AUG2 variant
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