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7p46
From Proteopedia
(Difference between revisions)
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==Crystal Structure of Xanthomonas campestris Tryptophan 2,3-dioxygenase (TDO)== | ==Crystal Structure of Xanthomonas campestris Tryptophan 2,3-dioxygenase (TDO)== | ||
| - | <StructureSection load='7p46' size='340' side='right'caption='[[7p46]]' scene=''> | + | <StructureSection load='7p46' size='340' side='right'caption='[[7p46]], [[Resolution|resolution]] 1.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P46 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7p46]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_campestris_pv._campestris_str._ATCC_33913 Xanthomonas campestris pv. campestris str. ATCC 33913]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P46 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p46 OCA], [https://pdbe.org/7p46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p46 RCSB], [https://www.ebi.ac.uk/pdbsum/7p46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p46 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=KYN:(2S)-2-AMINO-4-(2-AMINOPHENYL)-4-OXOBUTANOIC+ACID'>KYN</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p46 OCA], [https://pdbe.org/7p46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p46 RCSB], [https://www.ebi.ac.uk/pdbsum/7p46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p46 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/T23O_XANCP T23O_XANCP] Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring.[HAMAP-Rule:MF_01972]<ref>PMID:17197414</ref> <ref>PMID:18783250</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The kynurenine pathway is the major route of tryptophan metabolism. The first step of this pathway is catalysed by one of two heme-dependent dioxygenase enzymes - tryptophan 2,3-dioxygenase (TDO) and indoleamine 2,3-dioxygenase (IDO) - leading initially to the formation of N-formylkynurenine (NFK). In this paper, we present a crystal structure of a bacterial TDO from X. campestris in complex with l-kynurenine, the hydrolysed product of NFK. l-kynurenine is bound at the active site in a similar location to the substrate (l-Trp). Hydrogen bonding interactions with Arg117 and the heme 7-propionate anchor the l-kynurenine molecule into the pocket. A mechanism for the hydrolysis of NFK in the active site is presented. | ||
| + | |||
| + | Binding of l-kynurenine to X. campestris tryptophan 2,3-dioxygenase.,Basran J, Booth ES, Campbell LP, Thackray SJ, Jesani MH, Clayden J, Moody PCE, Mowat CG, Kwon H, Raven EL J Inorg Biochem. 2021 Sep 16;225:111604. doi: 10.1016/j.jinorgbio.2021.111604. PMID:34571402<ref>PMID:34571402</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7p46" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Dioxygenase 3D structures|Dioxygenase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Xanthomonas campestris pv. campestris str. ATCC 33913]] | ||
[[Category: Basran J]] | [[Category: Basran J]] | ||
[[Category: Booth ES]] | [[Category: Booth ES]] | ||
Current revision
Crystal Structure of Xanthomonas campestris Tryptophan 2,3-dioxygenase (TDO)
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