7p6v
From Proteopedia
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==N-TERMINAL BROMODOMAIN OF HUMAN BRD4 WITH compound 3ag== | ==N-TERMINAL BROMODOMAIN OF HUMAN BRD4 WITH compound 3ag== | ||
| - | <StructureSection load='7p6v' size='340' side='right'caption='[[7p6v]]' scene=''> | + | <StructureSection load='7p6v' size='340' side='right'caption='[[7p6v]], [[Resolution|resolution]] 1.17Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P6V FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P6V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P6V FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p6v OCA], [https://pdbe.org/7p6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p6v RCSB], [https://www.ebi.ac.uk/pdbsum/7p6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p6v ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.17Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5Z4:ethyl+2-(2-(4-azido-N-((2-(1,5-dimethyl-6-oxo-1,6-dihydropyridin-3-yl)-1-((tetrahydro-2H-pyran-4-yl)methyl)-1H-benzo[d]imidazol-6-yl)methyl)-2,3,5,6-tetrafluorobenzamido)acetamido)acetate'>5Z4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p6v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p6v OCA], [https://pdbe.org/7p6v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p6v RCSB], [https://www.ebi.ac.uk/pdbsum/7p6v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p6v ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We present a one-step Ugi reaction protocol for the expedient synthesis of photoaffinity probes for live-cell MS-based proteomics. The reaction couples an amine affinity function with commonly used photoreactive groups, and a variety of handle functionalities. Using this technology, a series of pan-BET (BET: bromodomain and extra-terminal domain) selective bromodomain photoaffinity probes were obtained by parallel synthesis. Studies on the effects of photoreactive group, linker length and irradiation wavelength on photocrosslinking efficiency provide valuable insights into photoaffinity probe design. Optimal probes were progressed to MS-based proteomics to capture the BET family of proteins from live cells and reveal their potential on- and off-target profiles. | ||
| + | |||
| + | One-Step Synthesis of Photoaffinity Probes for Live-Cell MS-Based Proteomics.,Fallon DJ, Lehmann S, Chung CW, Phillipou A, Eberl C, Fantom KGM, Zappacosta F, Patel VK, Bantscheff M, Schofield CJ, Tomkinson NCO, Bush JT Chemistry. 2021 Jul 30. doi: 10.1002/chem.202102036. PMID:34328642<ref>PMID:34328642</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7p6v" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Bromodomain-containing protein 3D structures|Bromodomain-containing protein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chung C]] | [[Category: Chung C]] | ||
Current revision
N-TERMINAL BROMODOMAIN OF HUMAN BRD4 WITH compound 3ag
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