7nxp
From Proteopedia
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==Structure of the C-terminal domain of the pUL77 capsid protein from human cytomegalovirus (HCMV)== | ==Structure of the C-terminal domain of the pUL77 capsid protein from human cytomegalovirus (HCMV)== | ||
| - | <StructureSection load='7nxp' size='340' side='right'caption='[[7nxp]]' scene=''> | + | <StructureSection load='7nxp' size='340' side='right'caption='[[7nxp]], [[Resolution|resolution]] 1.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NXP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7nxp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_betaherpesvirus_5 Human betaherpesvirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NXP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nxp OCA], [https://pdbe.org/7nxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nxp RCSB], [https://www.ebi.ac.uk/pdbsum/7nxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nxp ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.896Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nxp OCA], [https://pdbe.org/7nxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nxp RCSB], [https://www.ebi.ac.uk/pdbsum/7nxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nxp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CVC2_HCMVA CVC2_HCMVA] Capsid vertex-specific component that plays a role during viral DNA encapsidation, assuring correct genome cleavage and presumably stabilizing capsids that contain full-length viral genomes. Participates in the interaction between the capsid and the tegument through interaction with the large tegument protein/LTP.[HAMAP-Rule:MF_04025] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Herpesviruses cause severe diseases particularly in immunocompromised patients. Both genome packaging and release from the capsid require a unique portal channel occupying one of the 12 capsid vertices. Here, we report the 2.6 A crystal structure of the pentameric pORF19 of the gamma-herpesvirus Kaposi's sarcoma-associated herpesvirus (KSHV) resembling the portal cap that seals this portal channel. We also present the structure of its beta-herpesviral ortholog, revealing a striking structural similarity to its alpha- and gamma-herpesviral counterparts despite apparent differences in capsid association. We demonstrate pORF19 pentamer formation in solution and provide insights into how pentamerization is triggered in infected cells. Mutagenesis in its lateral interfaces blocked pORF19 pentamerization and severely affected KSHV capsid assembly and production of infectious progeny. Our results pave the way to better understand the role of pORF19 in capsid assembly and identify a potential novel drug target for the treatment of herpesvirus-induced diseases. | ||
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| + | Assembly of infectious Kaposi's sarcoma-associated herpesvirus progeny requires formation of a pORF19 pentamer.,Naniima P, Naimo E, Koch S, Curth U, Alkharsah KR, Stroh LJ, Binz A, Beneke JM, Vollmer B, Boning H, Borst EM, Desai P, Bohne J, Messerle M, Bauerfeind R, Legrand P, Sodeik B, Schulz TF, Krey T PLoS Biol. 2021 Nov 4;19(11):e3001423. doi: 10.1371/journal.pbio.3001423., eCollection 2021 Nov. PMID:34735435<ref>PMID:34735435</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7nxp" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human betaherpesvirus 5]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Krey T]] | [[Category: Krey T]] | ||
[[Category: Legrand P]] | [[Category: Legrand P]] | ||
[[Category: Naniima P]] | [[Category: Naniima P]] | ||
Current revision
Structure of the C-terminal domain of the pUL77 capsid protein from human cytomegalovirus (HCMV)
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