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1c9b

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CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)

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Retrieved from "http://52.214.119.220/wiki/index.php/1c9b"

Categories: Homo sapiens | Large Structures | Sigler PB | Tsai FTF

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-[[Image:1c9b.gif|left|200px]]<br />
-<applet load="1c9b" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1c9b, resolution 2.65&Aring;" />
-'''CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)==
-Transcription initiation requires the assembly of a preinitiation complex, (PIC), which is nucleated through binding of the TATA-box binding protein, (TBP) to the promoter. Biochemical studies have shown, however, that TBP, recognizes the TATA-box in both orientations and, therefore, cannot, account for the directionality of PIC assembly. Transcription factor IIB, (TFIIB) is essential for transcription initiation from RNA polymerase II, promoters. Recent functional studies have identified a specific 7 bp TFIIB, recognition element (BRE) immediately upstream of the TATA-box. We present, here the 2.65 A resolution crystal structure of a human TFIIBc-TBPc, complex bound to an idealized and extended adenovirus major late promoter., This structure now reveals that human TFIIBc binds to the promoter, asymmetrically through base-specific contacts in the major groove upstream, and in the minor groove downstream of the TATA-box. Binding of TFIIBc is, therefore, synergistic with TBPc requiring the distortion of the TATA-box., Thus, the newly described TFIIBc-DNA interface is likely to be a key, determinant for the unidirectional assembly of a functional PIC.
+<StructureSection load='1c9b' size='340' side='right'caption='[[1c9b]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1c9b]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C9B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C9B FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c9b OCA], [https://pdbe.org/1c9b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c9b RCSB], [https://www.ebi.ac.uk/pdbsum/1c9b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c9b ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/TF2B_HUMAN TF2B_HUMAN] General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/1c9b_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c9b ConSurf].
+<div style="clear:both"></div>
-==Disease==
+==See Also==
-Known diseases associated with this structure: Huntington disease-like-4 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600075 600075]], Parkinson disease OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600075 600075]], Spinocerebellar ataxia 17 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600075 600075]]
+*[[TATA-binding protein 3D structures|TATA-binding protein 3D structures]]
+*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
-==About this Structure==
+__TOC__
-C9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C9B OCA].
+</StructureSection>
-==Reference==
-Structural basis of preinitiation complex assembly on human pol II promoters., Tsai FT, Sigler PB, EMBO J. 2000 Jan 4;19(1):25-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10619841 10619841]
 [[Category: Homo sapiens]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Sigler, P.B.]]
+[[Category: Sigler PB]]
-[[Category: Tsai, F.T.F.]]
+[[Category: Tsai FTF]]
-[[Category: cyclin-like fold]]
-[[Category: helix-turn-helix]]
-[[Category: protein-dna complex]]
-[[Category: tata-box]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:18:37 2007''

1c9b

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Current revision

CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)

Structural highlights

Function

Evolutionary Conservation

See Also

Contents

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