7dhw

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of myosin-XI motor domain in complex with ADP-ALF4

Structural highlights

7dhw is a 1 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.84Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MYO6_ARATH Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Involved in the tip growth of root hair cells. Plays a major role in trafficking of Golgi stacks, mitochondria and peroxisomes during root hair development. Targets the peroxisome through an interaction with RABC2A. Required for development of pavement cells, trichomes, and stigmatic papillae.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

Publication Abstract from PubMed

Cytoplasmic streaming with extremely high velocity ( approximately 70 mum s(-1)) occurs in cells of the characean algae (Chara). Because cytoplasmic streaming is caused by myosin XI, it has been suggested that a myosin XI with a velocity of 70 mum s(-1), the fastest myosin measured so far, exists in Chara cells. However, the velocity of the previously cloned Chara corallina myosin XI (CcXI) was about 20 mum s(-1), one-third of the cytoplasmic streaming velocity in Chara Recently, the genome sequence of Chara braunii has been published, revealing that this alga has four myosin XI genes. We cloned these four myosin XI (CbXI-1, 2, 3, and 4) and measured their velocities. While the velocities of CbXI-3 and CbXI-4 motor domains (MDs) were similar to that of CcXI MD, the velocities of CbXI-1 and CbXI-2 MDs were 3.2 times and 2.8 times faster than that of CcXI MD, respectively. The velocity of chimeric CbXI-1, a functional, full-length CbXI-1 construct, was 60 mum s(-1) These results suggest that CbXI-1 and CbXI-2 would be the main contributors to cytoplasmic streaming in Chara cells and show that these myosins are ultrafast myosins with a velocity 10 times faster than fast skeletal muscle myosins in animals. We also report an atomic structure (2.8-A resolution) of myosin XI using X-ray crystallography. Based on this crystal structure and the recently published cryo-electron microscopy structure of acto-myosin XI at low resolution (4.3-A), it appears that the actin-binding region contributes to the fast movement of Chara myosin XI. Mutation experiments of actin-binding surface loops support this hypothesis.

Discovery of ultrafast myosin, its amino acid sequence, and structural features.,Haraguchi T, Tamanaha M, Suzuki K, Yoshimura K, Imi T, Tominaga M, Sakayama H, Nishiyama T, Murata T, Ito K Proc Natl Acad Sci U S A. 2022 Feb 22;119(8). pii: 2120962119. doi:, 10.1073/pnas.2120962119. PMID:35173046^[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hashimoto K, Igarashi H, Mano S, Nishimura M, Shimmen T, Yokota E. Peroxisomal localization of a myosin XI isoform in Arabidopsis thaliana. Plant Cell Physiol. 2005 May;46(5):782-9. doi: 10.1093/pcp/pci085. Epub 2005 Mar , 25. PMID:15792961 doi:http://dx.doi.org/10.1093/pcp/pci085
↑ Peremyslov VV, Prokhnevsky AI, Avisar D, Dolja VV. Two class XI myosins function in organelle trafficking and root hair development in Arabidopsis. Plant Physiol. 2008 Mar;146(3):1109-16. doi: 10.1104/pp.107.113654. Epub 2008 Jan, 4. PMID:18178669 doi:http://dx.doi.org/10.1104/pp.107.113654
↑ Prokhnevsky AI, Peremyslov VV, Dolja VV. Overlapping functions of the four class XI myosins in Arabidopsis growth, root hair elongation, and organelle motility. Proc Natl Acad Sci U S A. 2008 Dec 16;105(50):19744-9. doi:, 10.1073/pnas.0810730105. Epub 2008 Dec 5. PMID:19060218 doi:http://dx.doi.org/10.1073/pnas.0810730105
↑ Avisar D, Abu-Abied M, Belausov E, Sadot E, Hawes C, Sparkes IA. A comparative study of the involvement of 17 Arabidopsis myosin family members on the motility of Golgi and other organelles. Plant Physiol. 2009 Jun;150(2):700-9. doi: 10.1104/pp.109.136853. Epub 2009 Apr, 15. PMID:19369591 doi:http://dx.doi.org/10.1104/pp.109.136853
↑ Peremyslov VV, Prokhnevsky AI, Dolja VV. Class XI myosins are required for development, cell expansion, and F-Actin organization in Arabidopsis. Plant Cell. 2010 Jun;22(6):1883-97. doi: 10.1105/tpc.110.076315. Epub 2010 Jun, 25. PMID:20581304 doi:http://dx.doi.org/10.1105/tpc.110.076315
↑ Avisar D, Abu-Abied M, Belausov E, Sadot E. Myosin XIK is a major player in cytoplasm dynamics and is regulated by two amino acids in its tail. J Exp Bot. 2012 Jan;63(1):241-9. doi: 10.1093/jxb/err265. Epub 2011 Sep 13. PMID:21914656 doi:http://dx.doi.org/10.1093/jxb/err265
↑ Ojangu EL, Tanner K, Pata P, Jarve K, Holweg CL, Truve E, Paves H. Myosins XI-K, XI-1, and XI-2 are required for development of pavement cells, trichomes, and stigmatic papillae in Arabidopsis. BMC Plant Biol. 2012 Jun 6;12:81. doi: 10.1186/1471-2229-12-81. PMID:22672737 doi:http://dx.doi.org/10.1186/1471-2229-12-81
↑ Haraguchi T, Tamanaha M, Suzuki K, Yoshimura K, Imi T, Tominaga M, Sakayama H, Nishiyama T, Murata T, Ito K. Discovery of ultrafast myosin, its amino acid sequence, and structural features. Proc Natl Acad Sci U S A. 2022 Feb 22;119(8). pii: 2120962119. doi:, 10.1073/pnas.2120962119. PMID:35173046 doi:http://dx.doi.org/10.1073/pnas.2120962119

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7dhw"

@@ Line 3: / Line 3: @@
 <StructureSection load='7dhw' size='340' side='right'caption='[[7dhw]], [[Resolution|resolution]] 2.84&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7dhw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DHW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7dhw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DHW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.84&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">XI-2, MYA2, At5g43900, F6B6.4 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dhw OCA], [https://pdbe.org/7dhw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dhw RCSB], [https://www.ebi.ac.uk/pdbsum/7dhw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dhw ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/MYO6_ARATH MYO6_ARATH]] Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Involved in the tip growth of root hair cells. Plays a major role in trafficking of Golgi stacks, mitochondria and peroxisomes during root hair development. Targets the peroxisome through an interaction with RABC2A. Required for development of pavement cells, trichomes, and stigmatic papillae.<ref>PMID:15792961</ref> <ref>PMID:18178669</ref> <ref>PMID:19060218</ref> <ref>PMID:19369591</ref> <ref>PMID:20581304</ref> <ref>PMID:21914656</ref> <ref>PMID:22672737</ref>
+[https://www.uniprot.org/uniprot/MYO6_ARATH MYO6_ARATH] Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Involved in the tip growth of root hair cells. Plays a major role in trafficking of Golgi stacks, mitochondria and peroxisomes during root hair development. Targets the peroxisome through an interaction with RABC2A. Required for development of pavement cells, trichomes, and stigmatic papillae.<ref>PMID:15792961</ref> <ref>PMID:18178669</ref> <ref>PMID:19060218</ref> <ref>PMID:19369591</ref> <ref>PMID:20581304</ref> <ref>PMID:21914656</ref> <ref>PMID:22672737</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cytoplasmic streaming with extremely high velocity ( approximately 70 mum s(-1)) occurs in cells of the characean algae (Chara). Because cytoplasmic streaming is caused by myosin XI, it has been suggested that a myosin XI with a velocity of 70 mum s(-1), the fastest myosin measured so far, exists in Chara cells. However, the velocity of the previously cloned Chara corallina myosin XI (CcXI) was about 20 mum s(-1), one-third of the cytoplasmic streaming velocity in Chara Recently, the genome sequence of Chara braunii has been published, revealing that this alga has four myosin XI genes. We cloned these four myosin XI (CbXI-1, 2, 3, and 4) and measured their velocities. While the velocities of CbXI-3 and CbXI-4 motor domains (MDs) were similar to that of CcXI MD, the velocities of CbXI-1 and CbXI-2 MDs were 3.2 times and 2.8 times faster than that of CcXI MD, respectively. The velocity of chimeric CbXI-1, a functional, full-length CbXI-1 construct, was 60 mum s(-1) These results suggest that CbXI-1 and CbXI-2 would be the main contributors to cytoplasmic streaming in Chara cells and show that these myosins are ultrafast myosins with a velocity 10 times faster than fast skeletal muscle myosins in animals. We also report an atomic structure (2.8-A resolution) of myosin XI using X-ray crystallography. Based on this crystal structure and the recently published cryo-electron microscopy structure of acto-myosin XI at low resolution (4.3-A), it appears that the actin-binding region contributes to the fast movement of Chara myosin XI. Mutation experiments of actin-binding surface loops support this hypothesis.
+Discovery of ultrafast myosin, its amino acid sequence, and structural features.,Haraguchi T, Tamanaha M, Suzuki K, Yoshimura K, Imi T, Tominaga M, Sakayama H, Nishiyama T, Murata T, Ito K Proc Natl Acad Sci U S A. 2022 Feb 22;119(8). pii: 2120962119. doi:, 10.1073/pnas.2120962119. PMID:35173046<ref>PMID:35173046</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 7dhw" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Myosin 3D Structures|Myosin 3D Structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
 [[Category: Large Structures]]
-[[Category: Haraguchi, T]]
+[[Category: Haraguchi T]]
-[[Category: Imi, T]]
+[[Category: Imi T]]
-[[Category: Ito, K]]
+[[Category: Ito K]]
-[[Category: Murata, T]]
+[[Category: Murata T]]
-[[Category: Nishiyama, T]]
+[[Category: Nishiyama T]]
-[[Category: Sakayama, H]]
+[[Category: Sakayama H]]
-[[Category: Suzuki, K]]
+[[Category: Suzuki K]]
-[[Category: Tamanaha, M]]
+[[Category: Tamanaha M]]
-[[Category: Tominaga, M]]
+[[Category: Tominaga M]]
-[[Category: Yoshimura, K]]
+[[Category: Yoshimura K]]
-[[Category: Atp binding]]
-[[Category: Atpase]]
-[[Category: Motor domain]]
-[[Category: Motor protein]]
-[[Category: Myosin]]

7dhw

From Proteopedia

Current revision

Crystal structure of myosin-XI motor domain in complex with ADP-ALF4

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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