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1w94

From Proteopedia

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Crystal Structure of Mil (Mth680), an archaeal Imp4-like protein

Structural highlights

1w94 is a 2 chain structure with sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BRIX_METTH Probably involved in the biogenesis of the ribosome.[HAMAP-Rule:MF_00699]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Proteins of the Imp4/Brix superfamily are involved in ribosomal RNA processing, an essential function in all cells. We report the first structure of an Imp4/Brix superfamily protein, the Mil (for Methanothermobacter thermautotrophicus Imp4-like) protein (gene product Mth680), from the archaeon M. thermautotrophicus. The amino- and carboxy-terminal halves of Mil show significant structural similarity to one another, suggesting an origin by means of an ancestral duplication. Both halves show the same fold as the anticodon-binding domain of class IIa aminoacyl-tRNA synthetases, with greater conservation seen in the N-terminal half. This structural similarity, together with the charge distribution in Mil, suggests that Imp4/Brix superfamily proteins could bind single-stranded segments of RNA along a concave surface formed by the N-terminal half of their beta-sheet and a central alpha-helix. The crystal structure of Mil is incompatible with the presence, in the Imp4/Brix domain, of a helix-turn-helix motif that was proposed to comprise the RNA-binding moiety of the Imp4/Brix proteins.

Crystal structure of Mil (Mth680): internal duplication and similarity between the Imp4/Brix domain and the anticodon-binding domain of class IIa aminoacyl-tRNA synthetases.,Ng CL, Waterman D, Koonin EV, Antson AA, Ortiz-Lombardia M EMBO Rep. 2005 Feb;6(2):140-6. PMID:15654320^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ng CL, Waterman D, Koonin EV, Antson AA, Ortiz-Lombardia M. Crystal structure of Mil (Mth680): internal duplication and similarity between the Imp4/Brix domain and the anticodon-binding domain of class IIa aminoacyl-tRNA synthetases. EMBO Rep. 2005 Feb;6(2):140-6. PMID:15654320

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1w94"

Categories: Large Structures | Methanothermobacter thermautotrophicus | Antson AA | Ng CL | Ortiz-Lombardia M

@@ Line 3: / Line 3: @@
 <StructureSection load='1w94' size='340' side='right'caption='[[1w94]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1w94]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"methanobacterium_thermoautotrophicus"_(sic)_zeikus_and_wolfe_1972 "methanobacterium thermoautotrophicus" (sic) zeikus and wolfe 1972]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W94 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1w94]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W94 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w94 OCA], [https://pdbe.org/1w94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w94 RCSB], [https://www.ebi.ac.uk/pdbsum/1w94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w94 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/BRIX_METTH BRIX_METTH] Probably involved in the biogenesis of the ribosome.[HAMAP-Rule:MF_00699]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 34: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Antson, A A]]
+[[Category: Methanothermobacter thermautotrophicus]]
-[[Category: Ng, C L]]
+[[Category: Antson AA]]
-[[Category: Ortiz-Lombardia, M]]
+[[Category: Ng CL]]
-[[Category: Archaeal imp4-brix domain]]
+[[Category: Ortiz-Lombardia M]]
-[[Category: Brix domain]]
-[[Category: Imp4 domain]]
-[[Category: Rna-binding protein]]

1w94

From Proteopedia

Current revision

Crystal Structure of Mil (Mth680), an archaeal Imp4-like protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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