2j23

From Proteopedia

(Difference between revisions)

Current revision

Cross-reactivity and crystal structure of Malassezia sympodialis Thioredoxin (Mala s 13), a member of a new pan-allergen family

Structural highlights

2j23 is a 2 chain structure with sequence from Malassezia sympodialis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.41Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

THIO_MALSM Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We have identified thioredoxins (Trx) of Malassezia sympodialis, a yeast involved in the pathogenesis of atopic eczema, and of Aspergillus fumigatus, a fungus involved in pulmonary complications, as novel IgE-binding proteins. We show that these Trx, including the human enzyme, represent cross-reactive structures recognized by serum IgE from individuals sensitized to M. sympodialis Trx. Moreover, all three proteins were able to elicit immediate-type allergic skin reactions in sensitized individuals, indicating a humoral immune response based on molecular mimicry. To analyze structural elements involved in these reactions, the three-dimensional structure of M. sympodialis Trx (Mala s 13) has been determined at 1.4-A resolution by x-ray diffraction analysis. The structure was solved by molecular replacement and refined to a crystallographic R factor of 14.0% and a free R factor of 16.8% and shows the typical Trx fold. Mala s 13 shares 45% sequence identity with human Trx and superposition of the solved Mala s 13 structure with those of human Trx reveals a high similarity with a root mean square deviation of 1.11 A for all Calpha atoms. In a detailed analysis of the molecular surface in combination with sequence alignment, we identified conserved solvent-exposed amino acids scattered over the surface in both structures which cluster to patches, thus forming putative conformational B cell epitopes potentially involved in IgE-mediated cross- and autoreactivity.

Cross-reactivity and 1.4-A crystal structure of Malassezia sympodialis thioredoxin (Mala s 13), a member of a new pan-allergen family.,Limacher A, Glaser AG, Meier C, Schmid-Grendelmeier P, Zeller S, Scapozza L, Crameri R J Immunol. 2007 Jan 1;178(1):389-96. PMID:17182577^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Limacher A, Glaser AG, Meier C, Schmid-Grendelmeier P, Zeller S, Scapozza L, Crameri R. Cross-reactivity and 1.4-A crystal structure of Malassezia sympodialis thioredoxin (Mala s 13), a member of a new pan-allergen family. J Immunol. 2007 Jan 1;178(1):389-96. PMID:17182577
↑ Glaser AG, Menz G, Kirsch AI, Zeller S, Crameri R, Rhyner C. Auto aspergillosis. Allergy. 2008 Dec;63(12):1617-23. PMID:19032234 doi:10.1111/j.1398-9995.2008.01777.x
↑ Limacher A, Glaser AG, Meier C, Schmid-Grendelmeier P, Zeller S, Scapozza L, Crameri R. Cross-reactivity and 1.4-A crystal structure of Malassezia sympodialis thioredoxin (Mala s 13), a member of a new pan-allergen family. J Immunol. 2007 Jan 1;178(1):389-96. PMID:17182577

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j23"

@@ Line 3: / Line 3: @@
 <StructureSection load='2j23' size='340' side='right'caption='[[2j23]], [[Resolution|resolution]] 1.41&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2j23]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_96803 Atcc 96803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J23 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2j23]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Malassezia_sympodialis Malassezia sympodialis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J23 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J23 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j23 OCA], [https://pdbe.org/2j23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j23 RCSB], [https://www.ebi.ac.uk/pdbsum/2j23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j23 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.41&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j23 OCA], [https://pdbe.org/2j23 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j23 RCSB], [https://www.ebi.ac.uk/pdbsum/2j23 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j23 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/THIO_MALSM THIO_MALSM] Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions.<ref>PMID:17182577</ref> <ref>PMID:19032234</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 11: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j2/2j23_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 32: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 96803]]
 [[Category: Large Structures]]
-[[Category: Crameri, R]]
+[[Category: Malassezia sympodialis]]
-[[Category: Glaser, A G]]
+[[Category: Crameri R]]
-[[Category: Limacher, A]]
+[[Category: Glaser AG]]
-[[Category: Meier, C]]
+[[Category: Limacher A]]
-[[Category: Scapozza, L]]
+[[Category: Meier C]]
-[[Category: Allergen]]
+[[Category: Scapozza L]]
-[[Category: Autoreactivity]]
-[[Category: Cross-reactivity]]
-[[Category: Epitope]]
-[[Category: Fungi]]
-[[Category: Ige]]
-[[Category: Immune protein]]
-[[Category: Malassezia sympodiali]]
-[[Category: Thioredoxin]]

2j23

From Proteopedia

Current revision

Cross-reactivity and crystal structure of Malassezia sympodialis Thioredoxin (Mala s 13), a member of a new pan-allergen family

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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