Journal:Acta Cryst D:S2059798321009633
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In this paper, we present the first structure of SorC DBDs bound to DNA duplexes. We show DBD structures of representatives of each subfamily, <scene name='89/895271/Cv/4'>DeoR</scene> and <scene name='89/895271/Cv/7'>CggR</scene>, and compare their binding mode, which is likely common to all SorC family members. | In this paper, we present the first structure of SorC DBDs bound to DNA duplexes. We show DBD structures of representatives of each subfamily, <scene name='89/895271/Cv/4'>DeoR</scene> and <scene name='89/895271/Cv/7'>CggR</scene>, and compare their binding mode, which is likely common to all SorC family members. | ||
| + | *<scene name='89/895271/Cv/4'>A cartoon representation of the bsDeoRDBD dimer bound to the DNA operator</scene>. First monomer is colored in red and the second one is in salmon. | ||
*<scene name='89/895271/Cv/7'>A cartoon representation of the bsCggRDBD dimer bound to the DNA operator</scene>. ''bs''CggRDBD comprises five α-helices and two β-strands, with a topology of four α-helices α1 (1–13), α2 (15–32), α3 (37–43) and α4 (48–61) followed by <scene name='89/895271/Cv/8'>a β-hairpin (called a wing) formed by β1 (64–66) and β2 (71–73)</scene> and continuing with a final α-helix (75–89) that links the N-terminal DBD to the C-terminal EBD (α-helices are colored in red, β-strands are in yellow and second monomer is in salmon). | *<scene name='89/895271/Cv/7'>A cartoon representation of the bsCggRDBD dimer bound to the DNA operator</scene>. ''bs''CggRDBD comprises five α-helices and two β-strands, with a topology of four α-helices α1 (1–13), α2 (15–32), α3 (37–43) and α4 (48–61) followed by <scene name='89/895271/Cv/8'>a β-hairpin (called a wing) formed by β1 (64–66) and β2 (71–73)</scene> and continuing with a final α-helix (75–89) that links the N-terminal DBD to the C-terminal EBD (α-helices are colored in red, β-strands are in yellow and second monomer is in salmon). | ||
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| + | *<scene name='89/895271/Cv/15'>Morph showing the conformational change of bsDeoR-DBD upon DNA binding</scene>. DNA-free ''bs''DeoRDBD is colored in pink and DNA-bound ''bs''DeoRDBD is in red. <jmol><jmolButton> | ||
| + | <script>if (_animating); anim pause;set echo bottom left; color echo white; font echo 20 sansserif;echo Animation Paused; else; anim resume; set echo off;endif;</script> | ||
| + | <text>Stop Animation</text> | ||
| + | </jmolButton></jmol> | ||
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| + | '''PDB references:''' DNA-binding domain of DeoR in complex with DNA operator, [[7bhy]]; DNA-binding domain of CggR in complex with DNA operator, [[7oyk]]. | ||
<b>References</b><br> | <b>References</b><br> | ||
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