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| | ==SOLUTION STRUCTURE OF THE UMUD' HOMODIMER== | | ==SOLUTION STRUCTURE OF THE UMUD' HOMODIMER== |
| - | <StructureSection load='1i4v' size='340' side='right'caption='[[1i4v]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1i4v' size='340' side='right'caption='[[1i4v]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1i4v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I4V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1i4v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I4V FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UMUD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i4v OCA], [https://pdbe.org/1i4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i4v RCSB], [https://www.ebi.ac.uk/pdbsum/1i4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i4v ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i4v OCA], [https://pdbe.org/1i4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i4v RCSB], [https://www.ebi.ac.uk/pdbsum/1i4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i4v ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/UMUD_ECOLI UMUD_ECOLI]] Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template.
| + | [https://www.uniprot.org/uniprot/UMUD_ECOLI UMUD_ECOLI] Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ferentz, A E]] | + | [[Category: Ferentz AE]] |
| - | [[Category: Wagner, G]] | + | [[Category: Wagner G]] |
| - | [[Category: Walker, G C]] | + | [[Category: Walker GC]] |
| - | [[Category: Autocatalytic cleavage]]
| + | |
| - | [[Category: Dna polymerase accessory protein]]
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| - | [[Category: Dna polymerase v]]
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| - | [[Category: Dna repair]]
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| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Lambda ci]]
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| - | [[Category: Lexa repressor]]
| + | |
| - | [[Category: Serine protease]]
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| - | [[Category: Serine-lysine dyad]]
| + | |
| - | [[Category: Signal peptidase]]
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| - | [[Category: Sos mutagenesis]]
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| - | [[Category: Sos response]]
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| Structural highlights
Function
UMUD_ECOLI Involved in UV protection and mutation. Essential for induced (or SOS) mutagenesis. May modify the DNA replication machinery to allow bypass synthesis across a damaged template.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
During the SOS response of Escherichia coli to DNA damage, the umuDC operon is induced, producing the trimeric protein complexes UmuD2C, a DNA damage checkpoint effector, and UmuD'2C (DNA polymerase V), which carries out translesion synthesis, the basis of 'SOS mutagenesis'. UmuD'2, the homodimeric component of DNA pol V, is produced from UmuD by RecA-facilitated self-cleavage, which removes the 24 N-terminal residues of UmuD. We report the solution structure of UmuD'2 (PDB ID 1I4V) and interactions within UmuD'-UmuD, a heterodimer inactive in translesion synthesis. The overall shape of UmuD'2 in solution differs substantially from the previously reported crystal structure, even though the topologies of the two structures are quite similar. Most significantly, the active site residues S60 and K97 do not point directly at one another in solution as they do in the crystal, suggesting that self-cleavage of UmuD might require RecA to assemble the active site. Structural differences between UmuD'2 and UmuD'- UmuD suggest that UmuD'2C and UmuD2C might achieve their different biological activities through distinct interactions with RecA and DNA pol III.
Converting a DNA damage checkpoint effector (UmuD2C) into a lesion bypass polymerase (UmuD'2C).,Ferentz AE, Walker GC, Wagner G EMBO J. 2001 Aug 1;20(15):4287-98. PMID:11483531[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ferentz AE, Walker GC, Wagner G. Converting a DNA damage checkpoint effector (UmuD2C) into a lesion bypass polymerase (UmuD'2C). EMBO J. 2001 Aug 1;20(15):4287-98. PMID:11483531 doi:10.1093/emboj/20.15.4287
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