1fje

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[[Image:1fje.gif|left|200px]]
 
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==SOLUTION STRUCTURE OF NUCLEOLIN RBD12 IN COMPLEX WITH SNRE RNA==
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The line below this paragraph, containing "STRUCTURE_1fje", creates the "Structure Box" on the page.
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<StructureSection load='1fje' size='340' side='right'caption='[[1fje]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1fje]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesocricetus_auratus Mesocricetus auratus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FJE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fje OCA], [https://pdbe.org/1fje PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fje RCSB], [https://www.ebi.ac.uk/pdbsum/1fje PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fje ProSAT]</span></td></tr>
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{{STRUCTURE_1fje| PDB=1fje | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NUCL_MESAU NUCL_MESAU] Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity).<ref>PMID:3409881</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fj/1fje_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fje ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the 28 kDa complex of the first two RNA binding domains (RBDs) of nucleolin (RBD12) with an RNA stem-loop that includes the nucleolin recognition element UCCCGA in the loop was determined by NMR spectroscopy. The structure of nucleolin RBD12 with the nucleolin recognition element (NRE) reveals that the two RBDs bind on opposite sides of the RNA loop, forming a molecular clamp that brings the 5' and 3' ends of the recognition sequence close together and stabilizing the stem-loop. The specific interactions observed in the structure explain the sequence specificity for the NRE sequence. Binding studies of mutant proteins and analysis of conserved residues support the proposed interactions. The mode of interaction of the protein with the RNA and the location of the putative NRE sites suggest that nucleolin may function as an RNA chaperone to prevent improper folding of the nascent pre-rRNA.
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'''SOLUTION STRUCTURE OF NUCLEOLIN RBD12 IN COMPLEX WITH SNRE RNA'''
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Molecular basis of sequence-specific recognition of pre-ribosomal RNA by nucleolin.,Allain FH, Bouvet P, Dieckmann T, Feigon J EMBO J. 2000 Dec 15;19(24):6870-81. PMID:11118222<ref>PMID:11118222</ref>
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==Overview==
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The structure of the 28 kDa complex of the first two RNA binding domains (RBDs) of nucleolin (RBD12) with an RNA stem-loop that includes the nucleolin recognition element UCCCGA in the loop was determined by NMR spectroscopy. The structure of nucleolin RBD12 with the nucleolin recognition element (NRE) reveals that the two RBDs bind on opposite sides of the RNA loop, forming a molecular clamp that brings the 5' and 3' ends of the recognition sequence close together and stabilizing the stem-loop. The specific interactions observed in the structure explain the sequence specificity for the NRE sequence. Binding studies of mutant proteins and analysis of conserved residues support the proposed interactions. The mode of interaction of the protein with the RNA and the location of the putative NRE sites suggest that nucleolin may function as an RNA chaperone to prevent improper folding of the nascent pre-rRNA.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1FJE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mesocricetus_auratus Mesocricetus auratus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FJE OCA].
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</div>
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<div class="pdbe-citations 1fje" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Molecular basis of sequence-specific recognition of pre-ribosomal RNA by nucleolin., Allain FH, Bouvet P, Dieckmann T, Feigon J, EMBO J. 2000 Dec 15;19(24):6870-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11118222 11118222]
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*[[Nucleolin|Nucleolin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mesocricetus auratus]]
[[Category: Mesocricetus auratus]]
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[[Category: Single protein]]
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[[Category: Allain FHT]]
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[[Category: Allain, F H.T.]]
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[[Category: Bouvet P]]
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[[Category: Bouvet, P.]]
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[[Category: Dieckmann T]]
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[[Category: Dieckmann, T.]]
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[[Category: Feigon J]]
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[[Category: Feigon, J.]]
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[[Category: Nucleolus]]
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[[Category: Rbd]]
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[[Category: Rna binding domain]]
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[[Category: Rna-protein complex]]
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[[Category: Rnp]]
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[[Category: Rrm]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:23:54 2008''
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Current revision

SOLUTION STRUCTURE OF NUCLEOLIN RBD12 IN COMPLEX WITH SNRE RNA

PDB ID 1fje

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