1k5j
From Proteopedia
(Difference between revisions)
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<StructureSection load='1k5j' size='340' side='right'caption='[[1k5j]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='1k5j' size='340' side='right'caption='[[1k5j]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1k5j]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1k5j]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. The January 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Importins'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_1 10.2210/rcsb_pdb/mom_2007_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1K5J FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k5j OCA], [https://pdbe.org/1k5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k5j RCSB], [https://www.ebi.ac.uk/pdbsum/1k5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k5j ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1k5j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k5j OCA], [https://pdbe.org/1k5j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1k5j RCSB], [https://www.ebi.ac.uk/pdbsum/1k5j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1k5j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/NUPL_XENLA NUPL_XENLA] Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Nucleoplasmin is an acidic protein which is able to assemble nucleosomes by binding histones and transferring them to DNA. | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k5j ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1k5j ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The efficient assembly of histone complexes and nucleosomes requires the participation of molecular chaperones. Currently, there is a paucity of data on their mechanism of action. We now present the structure of an N-terminal domain of nucleoplasmin (Np-core) at 2.3 A resolution. The Np-core monomer is an eight-stranded beta barrel that fits snugly within a stable pentamer. In the crystal, two pentamers associate to form a decamer. We show that both Np and Np-core are competent to assemble large complexes that contain the four core histones. Further experiments and modeling suggest that these complexes each contain five histone octamers which dock to a central Np decamer. This work has important ramifications for models of histone storage, sperm chromatin decondensation, and nucleosome assembly. | ||
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| - | The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly.,Dutta S, Akey IV, Dingwall C, Hartman KL, Laue T, Nolte RT, Head JF, Akey CW Mol Cell. 2001 Oct;8(4):841-53. PMID:11684019<ref>PMID:11684019</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1k5j" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Nucleoplasmin|Nucleoplasmin]] | *[[Nucleoplasmin|Nucleoplasmin]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: African clawed frog]] | ||
[[Category: Importins]] | [[Category: Importins]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: RCSB PDB Molecule of the Month]] | [[Category: RCSB PDB Molecule of the Month]] | ||
| - | [[Category: Akey | + | [[Category: Xenopus laevis]] |
| - | [[Category: Akey | + | [[Category: Akey CW]] |
| - | [[Category: Dingwall | + | [[Category: Akey IV]] |
| - | [[Category: Dutta | + | [[Category: Dingwall C]] |
| - | [[Category: Hartman | + | [[Category: Dutta S]] |
| - | [[Category: Head | + | [[Category: Hartman KL]] |
| - | [[Category: Laue | + | [[Category: Head JF]] |
| - | [[Category: Nolte | + | [[Category: Laue T]] |
| - | + | [[Category: Nolte RT]] | |
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Current revision
The Crystal Structure of Nucleoplasmin-Core
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