1t3j
From Proteopedia
(Difference between revisions)
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<StructureSection load='1t3j' size='340' side='right'caption='[[1t3j]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='1t3j' size='340' side='right'caption='[[1t3j]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1t3j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[1t3j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T3J FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t3j OCA], [https://pdbe.org/1t3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t3j RCSB], [https://www.ebi.ac.uk/pdbsum/1t3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t3j ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t3j OCA], [https://pdbe.org/1t3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t3j RCSB], [https://www.ebi.ac.uk/pdbsum/1t3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t3j ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/MFN1_MOUSE MFN1_MOUSE] Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.<ref>PMID:12527753</ref> <ref>PMID:15297672</ref> | |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t3j ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t3j ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Vesicle fusion involves vesicle tethering, docking, and membrane merger. We show that mitofusin, an integral mitochondrial membrane protein, is required on adjacent mitochondria to mediate fusion, which indicates that mitofusin complexes act in trans (that is, between adjacent mitochondria). A heptad repeat region (HR2) mediates mitofusin oligomerization by assembling a dimeric, antiparallel coiled coil. The transmembrane segments are located at opposite ends of the 95 angstrom coiled coil and provide a mechanism for organelle tethering. Consistent with this proposal, truncated mitofusin, in an HR2-dependent manner, causes mitochondria to become apposed with a uniform gap. Our results suggest that HR2 functions as a mitochondrial tether before fusion. | ||
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| - | Structural basis of mitochondrial tethering by mitofusin complexes.,Koshiba T, Detmer SA, Kaiser JT, Chen H, McCaffery JM, Chan DC Science. 2004 Aug 6;305(5685):858-62. PMID:15297672<ref>PMID:15297672</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1t3j" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mus musculus]] |
| - | [[Category: Chan | + | [[Category: Chan DC]] |
| - | [[Category: Chen | + | [[Category: Chen H]] |
| - | [[Category: Detmer | + | [[Category: Detmer SA]] |
| - | [[Category: Kaiser | + | [[Category: Kaiser JT]] |
| - | [[Category: Koshiba | + | [[Category: Koshiba T]] |
| - | [[Category: McCaffery | + | [[Category: McCaffery JM]] |
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Current revision
Mitofusin domain HR2 V686M/I708M mutant
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Categories: Large Structures | Mus musculus | Chan DC | Chen H | Detmer SA | Kaiser JT | Koshiba T | McCaffery JM
