6yr5
From Proteopedia
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| - | ==== | + | ==14-3-3 sigma in complex with hDMX-367 peptide== |
| - | <StructureSection load='6yr5' size='340' side='right'caption='[[6yr5]]' scene=''> | + | <StructureSection load='6yr5' size='340' side='right'caption='[[6yr5]], [[Resolution|resolution]] 2.25Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yr5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YR5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yr5 OCA], [https://pdbe.org/6yr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yr5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yr5 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yr5 OCA], [https://pdbe.org/6yr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yr5 RCSB], [https://www.ebi.ac.uk/pdbsum/6yr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yr5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | p53 plays a critical role in regulating diverse biological processes: DNA repair, cell cycle arrest, apoptosis and senescence. The p53 pathway has therefore served as the focus of multiple drug-discovery efforts. p53 is negatively regulated by hDMX and hDM2; prior studies have identified 14-3-3 proteins as hDMX and hDM2 client proteins. 14-3-3 proteins are adaptor proteins that modulate localization, degradation and interactions of their targets in response to phosphorylation. Thus, 14-3-3 proteins may indirectly modulate the interaction between hDMX or hDM2 and p53 and represent potential targets for modulation of the p53 pathway. In this manuscript, we report on the biophysical and structural characterization of peptide/protein interactions that are representative of the interaction between 14-3-3 and hDMX or hDM2. The data establish that proximal phosphosites spaced ~20-25 residues apart in both hDMX and hDM2 co-operate to facilitate high-affinity 14-3-3 binding and provide structural insight that can be utilized in future stabilizer/inhibitor discovery efforts. | ||
| + | |||
| + | Understanding the interaction of 14-3-3 proteins with hDMX and hDM2: a structural and biophysical study.,Srdanovic S, Wolter M, Trinh CH, Ottmann C, Warriner SL, Wilson AJ FEBS J. 2022 Sep;289(17):5341-5358. doi: 10.1111/febs.16433. Epub 2022 Mar 28. PMID:35286747<ref>PMID:35286747</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6yr5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Ottman C]] |
| + | [[Category: Srdanovic S]] | ||
| + | [[Category: Warriner S]] | ||
| + | [[Category: Wilson A]] | ||
| + | [[Category: Wolter M]] | ||
Current revision
14-3-3 sigma in complex with hDMX-367 peptide
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