7m4s
From Proteopedia
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==Crystal structure of macrocyclase AMdnB from Anabaena sp. PCC 7120== | ==Crystal structure of macrocyclase AMdnB from Anabaena sp. PCC 7120== | ||
| - | <StructureSection load='7m4s' size='340' side='right'caption='[[7m4s]]' scene=''> | + | <StructureSection load='7m4s' size='340' side='right'caption='[[7m4s]], [[Resolution|resolution]] 2.49Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M4S FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M4S FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m4s OCA], [https://pdbe.org/7m4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m4s RCSB], [https://www.ebi.ac.uk/pdbsum/7m4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m4s ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.493Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m4s OCA], [https://pdbe.org/7m4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m4s RCSB], [https://www.ebi.ac.uk/pdbsum/7m4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m4s ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Microviridins, tricyclic peptide natural products originally isolated from cyanobacteria, function as inhibitors of diverse serine-type proteases. Here we report the structure and biochemical characterization of AMdnB, a unique iterative macrocyclase involved in a microviridin biosynthetic pathway from Anabaena sp. PCC 7120. The ATP-dependent cyclase, along with the homologous AMdnC, introduce up to nine macrocyclizations on three distinct core regions of a precursor peptide, AMdnA. The results presented here provide structural and mechanistic insight into the iterative chemistry of AMdnB. In vitro AMdnB-catalyzed cyclization reactions demonstrate the synthesis of the two predicted tricyclic products from a multi-core precursor peptide substrate, consistent with a distributive mode of catalysis. The X-ray structure of AMdnB shows a structural motif common to ATP-grasp cyclases involved in RiPPs biosynthesis. Additionally, comparison with the noniterative MdnB allows insight into the structural basis for the iterative chemistry. Overall, the presented results provide insight into the general mechanism of iterative enzymes in ribosomally synthesized and post-translationally modified peptide biosynthetic pathways. | ||
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| + | Structural and biochemical studies of an iterative ribosomal peptide macrocyclase.,Li G, Patel K, Zhang Y, Pugmire JK, Ding Y, Bruner SD Proteins. 2021 Oct 18. doi: 10.1002/prot.26264. PMID:34664307<ref>PMID:34664307</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7m4s" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of macrocyclase AMdnB from Anabaena sp. PCC 7120
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