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7nds
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==Crystal structure of TphC in a closed conformation== | ==Crystal structure of TphC in a closed conformation== | ||
| - | <StructureSection load='7nds' size='340' side='right'caption='[[7nds]]' scene=''> | + | <StructureSection load='7nds' size='340' side='right'caption='[[7nds]], [[Resolution|resolution]] 2.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NDS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7nds]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Comamonas_sp. Comamonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NDS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nds OCA], [https://pdbe.org/7nds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nds RCSB], [https://www.ebi.ac.uk/pdbsum/7nds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nds ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UB7:terephthalic+acid'>UB7</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nds OCA], [https://pdbe.org/7nds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nds RCSB], [https://www.ebi.ac.uk/pdbsum/7nds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nds ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A5N7XFM8_COMSP A0A5N7XFM8_COMSP] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Biological degradation of Polyethylene terephthalate (PET) plastic and assimilation of the corresponding monomers ethylene glycol and terephthalate (TPA) into central metabolism offers an attractive route for bio-based molecular recycling and bioremediation applications. A key step is the cellular uptake of the non-permeable TPA into bacterial cells which has been shown to be dependent upon the presence of the key tphC gene. However, little is known from a biochemical and structural perspective about the encoded solute binding protein, TphC. Here, we report the biochemical and structural characterisation of TphC in both open and TPA-bound closed conformations. This analysis demonstrates the narrow ligand specificity of TphC towards aromatic para-substituted dicarboxylates, such as TPA and closely related analogues. Further phylogenetic and genomic context analysis of the tph genes reveals homologous operons as a genetic resource for future biotechnological and metabolic engineering efforts towards circular plastic bio-economy solutions. | ||
| + | |||
| + | Structural basis of terephthalate recognition by solute binding protein TphC.,Gautom T, Dheeman D, Levy C, Butterfield T, Alvarez Gonzalez G, Le Roy P, Caiger L, Fisher K, Johannissen L, Dixon N Nat Commun. 2021 Oct 29;12(1):6244. doi: 10.1038/s41467-021-26508-0. PMID:34716322<ref>PMID:34716322</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7nds" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Comamonas sp]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Levy | + | [[Category: Levy C]] |
Current revision
Crystal structure of TphC in a closed conformation
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