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| | <StructureSection load='3guz' size='340' side='right'caption='[[3guz]], [[Resolution|resolution]] 1.67Å' scene=''> | | <StructureSection load='3guz' size='340' side='right'caption='[[3guz]], [[Resolution|resolution]] 1.67Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GUZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3guz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GUZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.67Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">panC ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PAF:PANTOATE'>PAF</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Pantoate--beta-alanine_ligase Pantoate--beta-alanine ligase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.1 6.3.2.1] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [https://pdbe.org/3guz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [https://www.ebi.ac.uk/pdbsum/3guz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3guz ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3guz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3guz OCA], [https://pdbe.org/3guz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3guz RCSB], [https://www.ebi.ac.uk/pdbsum/3guz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3guz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/PANC_ECOLI PANC_ECOLI]] Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.<ref>PMID:357689</ref>
| + | [https://www.uniprot.org/uniprot/PANC_ECOLI PANC_ECOLI] Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.<ref>PMID:357689</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Pantoate--beta-alanine ligase]]
| + | [[Category: Chakrabarti KS]] |
| - | [[Category: Chakrabarti, K S]] | + | [[Category: Gopal B]] |
| - | [[Category: Gopal, B]] | + | [[Category: Sarma SP]] |
| - | [[Category: Sarma, S P]] | + | [[Category: Thakur KG]] |
| - | [[Category: Thakur, K G]] | + | |
| - | [[Category: Atp-binding]]
| + | |
| - | [[Category: Competitive inhibition]]
| + | |
| - | [[Category: Cytoplasm]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Non-canonical pantoate binding-site]]
| + | |
| - | [[Category: Nucleotide-binding]]
| + | |
| - | [[Category: Pantothenate biosynthesis]]
| + | |
| - | [[Category: Rossmann fold]]
| + | |
| - | [[Category: Substrate binding]]
| + | |
| Structural highlights
Function
PANC_ECOLI Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The structural basis for the homotropic inhibition of pantothenate synthetase by the substrate pantoate was investigated by X-ray crystallography and high-resolution NMR spectroscopic methods. The tertiary structure of the dimeric N-terminal domain of Escherichia coli pantothenate synthetase, determined by X-ray crystallography to a resolution of 1.7 A, showed a second molecule of pantoate bound in the ATP-binding pocket. Pantoate binding to the ATP-binding site induced large changes in structure, mainly for backbone and side chain atoms of residues in the ATP binding HXGH(34-37) motif. Sequence-specific NMR resonance assignments and solution secondary structure of the dimeric N-terminal domain, obtained using samples enriched in (2)H, (13)C, and (15)N, indicated that the secondary structural elements were conserved in solution. Nitrogen-15 edited two-dimensional solution NMR chemical shift mapping experiments revealed that pantoate, at 10 mm, bound at these two independent sites. The solution NMR studies unambiguously demonstrated that ATP stoichiometrically displaced pantoate from the ATP-binding site. All NMR and X-ray studies were conducted at substrate concentrations used for enzymatic characterization of pantothenate synthetase from different sources [Jonczyk R & Genschel U (2006) J Biol Chem 281, 37435-37446]. As pantoate binding to its canonical site is structurally conserved, these results demonstrate that the observed homotropic effects of pantoate on pantothenate biosynthesis are caused by competitive binding of this substrate to the ATP-binding site. The results presented here have implications for the design and development of potential antibacterial and herbicidal agents.
X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate.,Chakrabarti KS, Thakur KG, Gopal B, Sarma SP FEBS J. 2010 Feb;277(3):697-712. Epub 2010 Jan 4. PMID:20059543[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Miyatake K, Nakano Y, Kitaoka S. Enzymological properties of pantothenate synthetase from Escherichia coli B. J Nutr Sci Vitaminol (Tokyo). 1978;24(3):243-53. PMID:357689
- ↑ Chakrabarti KS, Thakur KG, Gopal B, Sarma SP. X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate. FEBS J. 2010 Feb;277(3):697-712. Epub 2010 Jan 4. PMID:20059543 doi:10.1111/j.1742-4658.2009.07515.x
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