1foq

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[[Image:1foq.gif|left|200px]]
 
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==PENTAMERIC MODEL OF THE BACTERIOPHAGE PHI29 PROHEAD RNA==
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The line below this paragraph, containing "STRUCTURE_1foq", creates the "Structure Box" on the page.
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<SX load='1foq' size='340' side='right' viewer='molstar' caption='[[1foq]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1foq]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_virus_phi29 Bacillus virus phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FOQ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 20&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1foq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1foq OCA], [https://pdbe.org/1foq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1foq RCSB], [https://www.ebi.ac.uk/pdbsum/1foq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1foq ProSAT]</span></td></tr>
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{{STRUCTURE_1foq| PDB=1foq | SCENE= }}
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</table>
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<div style="background-color:#fffaf0;">
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'''PENTAMERIC MODEL OF THE BACTERIOPHAGE PHI29 PROHEAD RNA'''
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== Publication Abstract from PubMed ==
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==Overview==
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Motors generating mechanical force, powered by the hydrolysis of ATP, translocate double-stranded DNA into preformed capsids (proheads) of bacterial viruses and certain animal viruses. Here we describe the motor that packages the double-stranded DNA of the Bacillus subtilis bacteriophage phi29 into a precursor capsid. We determined the structure of the head-tail connector--the central component of the phi29 DNA packaging motor--to 3.2 A resolution by means of X-ray crystallography. We then fitted the connector into the electron densities of the prohead and of the partially packaged prohead as determined using cryo-electron microscopy and image reconstruction analysis. Our results suggest that the prohead plus dodecameric connector, prohead RNA, viral ATPase and DNA comprise a rotary motor with the head-prohead RNA-ATPase complex acting as a stator, the DNA acting as a spindle, and the connector as a ball-race. The helical nature of the DNA converts the rotary action of the connector into translation of the DNA.
Motors generating mechanical force, powered by the hydrolysis of ATP, translocate double-stranded DNA into preformed capsids (proheads) of bacterial viruses and certain animal viruses. Here we describe the motor that packages the double-stranded DNA of the Bacillus subtilis bacteriophage phi29 into a precursor capsid. We determined the structure of the head-tail connector--the central component of the phi29 DNA packaging motor--to 3.2 A resolution by means of X-ray crystallography. We then fitted the connector into the electron densities of the prohead and of the partially packaged prohead as determined using cryo-electron microscopy and image reconstruction analysis. Our results suggest that the prohead plus dodecameric connector, prohead RNA, viral ATPase and DNA comprise a rotary motor with the head-prohead RNA-ATPase complex acting as a stator, the DNA acting as a spindle, and the connector as a ball-race. The helical nature of the DNA converts the rotary action of the connector into translation of the DNA.
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==About this Structure==
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Structure of the bacteriophage phi29 DNA packaging motor.,Simpson AA, Tao Y, Leiman PG, Badasso MO, He Y, Jardine PJ, Olson NH, Morais MC, Grimes S, Anderson DL, Baker TS, Rossmann MG Nature. 2000 Dec 7;408(6813):745-50. PMID:11130079<ref>PMID:11130079</ref>
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1FOQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_phi29 Bacteriophage phi29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOQ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of the bacteriophage phi29 DNA packaging motor., Simpson AA, Tao Y, Leiman PG, Badasso MO, He Y, Jardine PJ, Olson NH, Morais MC, Grimes S, Anderson DL, Baker TS, Rossmann MG, Nature. 2000 Dec 7;408(6813):745-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11130079 11130079]
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</div>
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[[Category: Bacteriophage phi29]]
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<div class="pdbe-citations 1foq" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Anderson, D L.]]
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<references/>
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[[Category: Badasso, M O.]]
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__TOC__
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[[Category: Baker, T S.]]
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</SX>
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[[Category: Grimes, S.]]
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[[Category: Bacillus virus phi29]]
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[[Category: He, Y.]]
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[[Category: Large Structures]]
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[[Category: Jardine, P J.]]
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[[Category: Anderson DL]]
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[[Category: Leiman, P G.]]
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[[Category: Badasso MO]]
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[[Category: Morais, M C.]]
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[[Category: Baker TS]]
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[[Category: Olson, N H.]]
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[[Category: Grimes S]]
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[[Category: Rossmann, M G.]]
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[[Category: He Y]]
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[[Category: Simpson, A A.]]
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[[Category: Jardine PJ]]
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[[Category: Tao, Y.]]
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[[Category: Leiman PG]]
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[[Category: Bacteriophage phi29]]
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[[Category: Morais MC]]
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[[Category: Dsrna oligomeric model]]
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[[Category: Olson NH]]
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[[Category: Prohead rna]]
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[[Category: Rossmann MG]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:35:06 2008''
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[[Category: Simpson AA]]
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[[Category: Tao Y]]

Current revision

PENTAMERIC MODEL OF THE BACTERIOPHAGE PHI29 PROHEAD RNA

1foq, resolution 20.00Å

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