7s64
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Intermediate-form oocyte/egg Alpha-2-Macroglobulin (A2Moo) tetramer== | |
| + | <StructureSection load='7s64' size='340' side='right'caption='[[7s64]], [[Resolution|resolution]] 6.43Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7s64]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S64 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S64 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s64 OCA], [https://pdbe.org/7s64 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s64 RCSB], [https://www.ebi.ac.uk/pdbsum/7s64 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s64 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Alpha-2-Macroglobulin (A2M) is the critical pan-protease inhibitor of the innate immune system. When proteases cleave the A2M bait region, global structural transformation of the A2M tetramer is triggered to entrap the protease. The structural basis behind the cleavage-induced transformation and the protease entrapment remains unclear. Here, we report cryo-EM structures of native- and intermediate-forms of the Xenopus laevis egg A2M homolog (A2Moo or ovomacroglobulin) tetramer at 3.7-4.1 A and 6.4 A resolution, respectively. In the native A2Moo tetramer, two pairs of dimers arrange into a cross-like configuration with four 60 A-wide bait-exposing grooves. Each bait in the native form threads into an aperture formed by three macroglobulin domains (MG2, MG3, MG6). The bait is released from the narrowed aperture in the induced protomer of the intermediate form. We propose that the intact bait region works as a "latch-lock" to block futile A2M transformation until its protease-mediated cleavage. | ||
| - | + | Structural Mechanics of the Alpha-2-Macroglobulin Transformation.,Arimura Y, Funabiki H J Mol Biol. 2021 Dec 20:167413. doi: 10.1016/j.jmb.2021.167413. PMID:34942166<ref>PMID:34942166</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7s64" style="background-color:#fffaf0;"></div> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Xenopus laevis]] | ||
[[Category: Arimura, Y]] | [[Category: Arimura, Y]] | ||
| + | [[Category: Funabiki, H]] | ||
| + | [[Category: Protease inhibitor]] | ||
| + | [[Category: Protein binding]] | ||
| + | [[Category: Xenopus egg extract]] | ||
Current revision
Intermediate-form oocyte/egg Alpha-2-Macroglobulin (A2Moo) tetramer
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