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| - | [[Image:1fpx.jpg|left|200px]] | + | #REDIRECT [[6cig]] This PDB entry is obsolete and replaced by 6cig |
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| - | {{STRUCTURE_1fpx| PDB=1fpx | SCENE= }}
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| - | '''CRYSTAL STRUCTURE ANALYSIS OF SELENOMETHIONINE SUBSTITUTED ISOFLAVONE O-METHYLTRANSFERASE'''
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| - | ==Overview==
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| - | Chalcone O-methyltransferase (ChOMT) and isoflavone O-methyltransferase (IOMT) are S-adenosyl-l-methionine (SAM) dependent plant natural product methyltransferases involved in secondary metabolism in Medicago sativa (alfalfa). Here we report the crystal structure of ChOMT in complex with the product S-adenosyl-l-homocysteine and the substrate isoliquiritigenin (4,2',4'-trihydroxychalcone) refined to 1.8 A as well as the crystal structure of IOMT in complex with the products S-adenosyl-l-homocysteine and isoformononetin (4'-hydroxy-7-methoxyisoflavone) refined to 1.4 A. These two OMTs constitute the first plant methyltransferases to be structurally characterized and reveal a novel oligomerization domain and the molecular determinants for substrate selection. As such, this work provides a structural basis for understanding the substrate specificity of the diverse family of plant OMTs and facilitates the engineering of novel activities in this extensive class of natural product biosynthetic enzymes.
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| - | ==About this Structure==
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| - | 1FPX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FPX OCA].
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| - | ==Reference==
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| - | Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases., Zubieta C, He XZ, Dixon RA, Noel JP, Nat Struct Biol. 2001 Mar;8(3):271-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11224575 11224575]
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| - | [[Category: Medicago sativa]]
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| - | [[Category: Single protein]]
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| - | [[Category: Dixon, R A.]]
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| - | [[Category: Noel, J P.]]
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| - | [[Category: Zubieta, C.]]
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| - | [[Category: S-adenosymethionine complex]]
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| - | [[Category: Selenomethionine]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:37:35 2008''
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