2yjg

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<StructureSection load='2yjg' size='340' side='right'caption='[[2yjg]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='2yjg' size='340' side='right'caption='[[2yjg]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum Thermoanaerobacterium thermosaccharolyticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YJG FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2yjg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobacterium_thermosaccharolyticum_DSM_571 Thermoanaerobacterium thermosaccharolyticum DSM 571]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YJG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lactate_racemase Lactate racemase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.2.1 5.1.2.1] </span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [https://pdbe.org/2yjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [https://www.ebi.ac.uk/pdbsum/2yjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjg ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yjg OCA], [https://pdbe.org/2yjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yjg RCSB], [https://www.ebi.ac.uk/pdbsum/2yjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yjg ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/LARA_THETC LARA_THETC] Catalyzes the interconversion between the D- and L-isomers of lactate.<ref>PMID:24710389</ref>
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Racemases catalyse the inversion of stereochemistry in biological molecules, giving the organism the ability to use both isomers. Among them, lactate racemase remains unexplored due to its intrinsic instability and lack of molecular characterization. Here we determine the genetic basis of lactate racemization in Lactobacillus plantarum. We show that, unexpectedly, the racemase is a nickel-dependent enzyme with a novel alpha/beta fold. In addition, we decipher the process leading to an active enzyme, which involves the activation of the apo-enzyme by a single nickel-containing maturation protein that requires preactivation by two other accessory proteins. Genomic investigations reveal the wide distribution of the lactate racemase system among prokaryotes, showing the high significance of both lactate enantiomers in carbon metabolism. The even broader distribution of the nickel-based maturation system suggests a function beyond activation of the lactate racemase and possibly linked with other undiscovered nickel-dependent enzymes.
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Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system.,Desguin B, Goffin P, Viaene E, Kleerebezem M, Martin-Diaconescu V, Maroney MJ, Declercq JP, Soumillion P, Hols P Nat Commun. 2014 Apr 7;5:3615. doi: 10.1038/ncomms4615. PMID:24710389<ref>PMID:24710389</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2yjg" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Lactate racemase]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Thermoanaerobacterium thermosaccharolyticum]]
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[[Category: Thermoanaerobacterium thermosaccharolyticum DSM 571]]
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[[Category: Declercq, J P]]
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[[Category: Declercq JP]]
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[[Category: Desguin, B]]
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[[Category: Desguin B]]
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[[Category: Hols, P]]
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[[Category: Hols P]]
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[[Category: Soumillion, P]]
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[[Category: Soumillion P]]
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[[Category: Isomerase]]
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[[Category: Nickel-dependent enzyme]]
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Current revision

Structure of the lactate racemase apoprotein from Thermoanaerobacterium thermosaccharolyticum

PDB ID 2yjg

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