7qac
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The T2 structure of polycrystalline cubic human insulin== |
| + | <StructureSection load='7qac' size='340' side='right'caption='[[7qac]], [[Resolution|resolution]] 2.29Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7qac]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QAC FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray powder diffraction, [[Resolution|Resolution]] 2.29Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qac OCA], [https://pdbe.org/7qac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qac RCSB], [https://www.ebi.ac.uk/pdbsum/7qac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qac ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The polymorphism of human insulin upon pH variation was characterized via X-ray powder diffraction, employing a crystallization protocol previously established for co-crystallization with phenolic derivatives. Two distinct rhombohedral (R3) polymorphs and one cubic (I2(1)3) polymorph were identified with increasing pH, corresponding to the T(6), T(3)R(3)(f) and T(2) conformations of insulin, respectively. The structure of the cubic T(2) polymorph was determined via multi-profile stereochemically restrained Rietveld refinement at 2.7 A resolution. This constitutes the first cubic insulin structure to be determined from crystals grown in the presence of zinc ions, although no zinc binding was observed. The differences of the polycrystalline variant from other cubic insulin structures, as well as the nature of the pH-driven phase transitions, are discussed in detail. | ||
| - | + | The T(2) structure of polycrystalline cubic human insulin.,Triandafillidis DP, Karavassili F, Spiliopoulou M, Valmas A, Athanasiadou M, Nikolaras G, Fili S, Kontou P, Bowler MW, Chasapis CT, Von Dreele RB, Fitch AN, Margiolaki I Acta Crystallogr D Struct Biol. 2023 May 1;79(Pt 5):374-386. doi: , 10.1107/S2059798323001328. Epub 2023 Apr 11. PMID:37039669<ref>PMID:37039669</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7qac" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bowler MW]] | ||
| + | [[Category: Fili S]] | ||
| + | [[Category: Fitch A]] | ||
| + | [[Category: Karavassili F]] | ||
| + | [[Category: Kontou P]] | ||
| + | [[Category: Margiolaki I]] | ||
| + | [[Category: Spiliopoulou M]] | ||
| + | [[Category: Triandafillidis DP]] | ||
| + | [[Category: Valmas A]] | ||
| + | [[Category: Von Dreele RB]] | ||
Current revision
The T2 structure of polycrystalline cubic human insulin
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