1fr3

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[[Image:1fr3.gif|left|200px]]
 
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==THE HIGH RESOLUTION STRUCTURE OF A MOLYBDATE BINDING PROTEIN FROM SPOROMUSA OVATA==
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The line below this paragraph, containing "STRUCTURE_1fr3", creates the "Structure Box" on the page.
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<StructureSection load='1fr3' size='340' side='right'caption='[[1fr3]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1fr3]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Sporomusa_ovata Sporomusa ovata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FR3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FR3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
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{{STRUCTURE_1fr3| PDB=1fr3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fr3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fr3 OCA], [https://pdbe.org/1fr3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fr3 RCSB], [https://www.ebi.ac.uk/pdbsum/1fr3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fr3 ProSAT]</span></td></tr>
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</table>
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'''THE HIGH RESOLUTION STRUCTURE OF A MOLYBDATE BINDING PROTEIN FROM SPOROMUSA OVATA'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q7SIF7_9FIRM Q7SIF7_9FIRM]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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BACKGROUND: Transport of molybdenum into bacteria involves a high-affinity ABC transporter system whose expression is controlled by a repressor protein called ModE. While molybdate transport is tightly coupled to utilization in some bacteria, other organisms have molybdenum storage proteins. One class of putative molybdate storage proteins is characterized by a sequence consisting of about 70 amino acids (Mop). A tandem repeat of Mop sequences also constitutes the molybdate binding domain of ModE. RESULTS: We have determined the crystal structure of the 7 kDa Mop protein from the methanol-utilizing anaerobic eubacterium Sporomusa ovata grown in the presence of molybdate and tungstate. The protein occurs as highly symmetric hexamers binding eight oxyanions. Each peptide assumes a so-called OB fold, which has previously also been observed in ModE. There are two types of oxyanion binding sites in Mo at the interface between two or three peptides. All oxyanion binding sites were found to be occupied by WO(4) rather than MoO(4). CONCLUSIONS: The biological function of proteins containing only Mop sequences is unknown, but they have been implicated in molybdate homeostasis and molybdopterin cofactor biosynthesis. While there are few indications that the S. ovata Mop binds pterin, the structure suggests that only the type-1 oxyanion binding sites would be sufficiently accessible to bind a cofactor. The observed occupation of the oxyanion binding sites by WO(4) indicates that Mop might also be involved in controlling intracellular tungstate levels.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fr/1fr3_consurf.spt"</scriptWhenChecked>
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1FR3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sporomusa_ovata Sporomusa ovata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FR3 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of the molybdate/tungstate binding protein mop from Sporomusa ovata., Wagner UG, Stupperich E, Kratky C, Structure. 2000 Nov 15;8(11):1127-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11080635 11080635]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fr3 ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sporomusa ovata]]
[[Category: Sporomusa ovata]]
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[[Category: Kratky, C.]]
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[[Category: Kratky C]]
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[[Category: Stupperich, E.]]
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[[Category: Stupperich E]]
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[[Category: Wagner, U G.]]
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[[Category: Wagner UG]]
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[[Category: Molybdate]]
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[[Category: Molybdate homeostasis]]
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[[Category: Tungstate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:40:16 2008''
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Current revision

THE HIGH RESOLUTION STRUCTURE OF A MOLYBDATE BINDING PROTEIN FROM SPOROMUSA OVATA

PDB ID 1fr3

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