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7vxq
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The Carbon Monoxide Complex of [NiFe]-hydrogenase (Hyb-type) from Citrobacter sp. S-77== |
| + | <StructureSection load='7vxq' size='340' side='right'caption='[[7vxq]], [[Resolution|resolution]] 1.77Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7vxq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_sp._S-77 Citrobacter sp. S-77]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VXQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.77Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=F3S:FE3-S4+CLUSTER'>F3S</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NFU:FORMYL[BIS(HYDROCYANATO-1KAPPAC)]IRONNICKEL(FE-NI)'>NFU</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vxq OCA], [https://pdbe.org/7vxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vxq RCSB], [https://www.ebi.ac.uk/pdbsum/7vxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vxq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A3B6UEQ1_9ENTR A0A3B6UEQ1_9ENTR] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hydrogenases catalyze the reversible oxidation of H(2). Carbon monoxide (CO) is known to be a competitive inhibitor of O(2)-sensitive [NiFe]-hydrogenases. Although the activities of some O(2)-tolerant [NiFe]-hydrogenases are unaffected by CO, the partially O(2)-tolerant [NiFe]-hydrogenase from Citrobacter sp. S-77 (S77-HYB) is inhibited by CO. In this work, the CO-bound state of S77-HYB was characterized by activity assays, spectroscopic techniques and X-ray crystallography. Electron paramagnetic resonance spectroscopy showed a diamagnetic Ni(2+) state, and Fourier-transform infrared spectroscopy revealed the stretching vibration of the exogenous CO ligand. The crystal structure determined at 1.77 A resolution revealed that CO binds weakly to the nickel ion in the Ni-Fe active site of S77-HYB. These results suggest a positive correlation between O(2) and CO tolerance in [NiFe]-hydrogenases. | ||
| - | + | Structural and spectroscopic characterization of CO inhibition of [NiFe]-hydrogenase from Citrobacter sp. S-77.,Imanishi T, Nishikawa K, Taketa M, Higuchi K, Tai H, Hirota S, Hojo H, Kawakami T, Hataguchi K, Matsumoto K, Ogata H, Higuchi Y Acta Crystallogr F Struct Biol Commun. 2022 Feb 1;78(Pt 2):66-74. doi: , 10.1107/S2053230X22000188. Epub 2022 Jan 27. PMID:35102895<ref>PMID:35102895</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7vxq" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Citrobacter sp. S-77]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Higuchi K]] | ||
| + | [[Category: Higuchi Y]] | ||
| + | [[Category: Imanishi T]] | ||
| + | [[Category: Nishikawa K]] | ||
Current revision
The Carbon Monoxide Complex of [NiFe]-hydrogenase (Hyb-type) from Citrobacter sp. S-77
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