7oz6
From Proteopedia
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<StructureSection load='7oz6' size='340' side='right'caption='[[7oz6]], [[Resolution|resolution]] 1.76Å' scene=''> | <StructureSection load='7oz6' size='340' side='right'caption='[[7oz6]], [[Resolution|resolution]] 1.76Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OZ6 FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.757Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oz6 OCA], [https://pdbe.org/7oz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oz6 RCSB], [https://www.ebi.ac.uk/pdbsum/7oz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oz6 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oz6 OCA], [https://pdbe.org/7oz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oz6 RCSB], [https://www.ebi.ac.uk/pdbsum/7oz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oz6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Rhizobium etli, a nitrogen-fixing bacterial symbiont of legume plants, encodes an essential L-asparaginase (ReAV) with no sequence homology to known enzymes with this activity. High-resolution crystal structures of ReAV show indeed a structurally distinct, dimeric enzyme, with some resemblance to glutaminases and beta-lactamases. However, ReAV has no glutaminase or lactamase activity, and at pH 9 its allosteric asparaginase activity is relatively high, with Km for L-Asn at 4.2 mM and kcat of 438 s(-1). The active site of ReAV, deduced from structural comparisons and confirmed by mutagenesis experiments, contains a highly specific Zn(2+) binding site without a catalytic role. The extensive active site includes residues with unusual chemical properties. There are two Ser-Lys tandems, all connected through a network of H-bonds to the Zn center, and three tightly bound water molecules near Ser48, which clearly indicate the catalytic nucleophile. | ||
| - | + | ==See Also== | |
| - | + | *[[Asparaginase 3D structures|Asparaginase 3D structures]] | |
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Gilski | + | [[Category: Gilski M]] |
| - | [[Category: Imiolczyk | + | [[Category: Imiolczyk B]] |
| - | [[Category: Jaskolski | + | [[Category: Jaskolski M]] |
| - | [[Category: Loch | + | [[Category: Loch JI]] |
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Current revision
Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MC)
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