7rfd

<table><tr><td colspan='2'>[[7rfd]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RFD FirstGlance]. <br>

</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=55I:'>55I</scene></td></tr>

<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7n3j|7n3j]]</div></td></tr>

<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peptidylprolyl_isomerase Peptidylprolyl isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.2.1.8 5.2.1.8] </span></td></tr>

== Function ==

[[https://www.uniprot.org/uniprot/J7QN08_ECOLX J7QN08_ECOLX]] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.[RuleBase:RU363019]

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== Publication Abstract from PubMed ==

Fluorine atoms are known to display scalar (19)F-(19)F couplings in nuclear magnetic resonance (NMR) spectra when they are sufficiently close in space for nonbonding orbitals to overlap. We show that fluorinated noncanonical amino acids positioned in the hydrophobic core or on the surface of a protein can be linked by scalar through-space (19)F-(19)F ((TS)JFF) couplings even if the (19)F spins are in the time average separated by more than the van der Waals distance. Using two different aromatic amino acids featuring CF3 groups, O-trifluoromethyl-tyrosine and 4-trifluoromethyl-phenylalanine, we show that (19)F-(19)F TOCSY experiments are sufficiently sensitive to detect (TS)JFF couplings between 2.5 and 5 Hz in the 19 kDa protein PpiB measured on a two-channel 400 MHz NMR spectrometer with a regular room temperature probe. A quantitative J evolution experiment enables the measurement of (TS)JFF coupling constants that are up to five times smaller than the (19)F NMR line width. In addition, a new aminoacyl-tRNA synthetase was identified for genetic encoding of N(6)-(trifluoroacetyl)-l-lysine (TFA-Lys) and (19)F-(19)F TOCSY peaks were observed between two TFA-Lys residues incorporated into the proteins AncCDT-1 and mRFP despite high solvent exposure and flexibility of the TFA-Lys side chains. With the ready availability of systems for site-specific incorporation of fluorinated amino acids into proteins by genetic encoding, (19)F-(19)F interactions offer a straightforward way to probe the spatial proximity of selected sites without any assignments of (1)H NMR resonances.

 

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== References ==

[[Category: Frkic, R L]]

[[Category: Jackson, C J]]

[[Category: Otting, G]]

[[Category: Trifluoromethyl-phenylalanine]]