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| | ==Solution structure of an arsenate reductase (ArsC) related protein from Brucella melitensis. Seattle Structural Genomics Center for Infectious Disease target BrabA.00007.a.== | | ==Solution structure of an arsenate reductase (ArsC) related protein from Brucella melitensis. Seattle Structural Genomics Center for Infectious Disease target BrabA.00007.a.== |
| - | <StructureSection load='2kok' size='340' side='right'caption='[[2kok]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2kok' size='340' side='right'caption='[[2kok]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kok]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_abortus"_schmidt_in_schmidt_and_weis_1901 "bacterium abortus" schmidt in schmidt and weis 1901]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KOK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kok]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KOK FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ArsC, BruAb1_0395 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=235 "Bacterium abortus" Schmidt in Schmidt and Weis 1901])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kok OCA], [https://pdbe.org/2kok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kok RCSB], [https://www.ebi.ac.uk/pdbsum/2kok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kok ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kok OCA], [https://pdbe.org/2kok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kok RCSB], [https://www.ebi.ac.uk/pdbsum/2kok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kok ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q2YMA4_BRUA2 Q2YMA4_BRUA2] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacterium abortus schmidt in schmidt and weis 1901]] | + | [[Category: Brucella abortus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Buchko, G W]] | + | [[Category: Buchko GW]] |
| - | [[Category: Hewitt, S N]] | + | [[Category: Hewitt SN]] |
| - | [[Category: Napuli, A J]] | + | [[Category: Napuli AJ]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Van Voorhis WC]] |
| - | [[Category: Voorhis, W C.Van]] | + | |
| - | [[Category: Arsenate reductase]]
| + | |
| - | [[Category: Brucellosis]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Ssgcid]]
| + | |
| - | [[Category: Zoonotic]]
| + | |
| Structural highlights
Function
Q2YMA4_BRUA2
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Brucella melitensis is the etiological agent responsible for brucellosis. Present in the B. melitensis genome is a 116-residue protein related to arsenate reductases (Bm-YffB; BR0369). Arsenate reductases (ArsC) convert arsenate ion (H(2)AsO(4)(-)), a compound that is toxic to bacteria, to arsenite ion (AsO(2)(-)), a product that may be efficiently exported out of the cell. Consequently, Bm-YffB is a potential drug target because if arsenate reduction is the protein's major biological function then disabling the cell's ability to reduce arsenate would make these cells more sensitive to the deleterious effects of arsenate. Size-exclusion chromatography and NMR spectroscopy indicate that Bm-YffB is a monomer in solution. The solution structure of Bm-YffB (PDB entry 2kok) shows that the protein consists of two domains: a four-stranded mixed beta-sheet flanked by two alpha-helices on one side and an alpha-helical bundle. The alpha/beta domain is characteristic of the fold of thioredoxin-like proteins and the overall structure is generally similar to those of known arsenate reductases despite the marginal sequence similarity. Chemical shift perturbation studies with (15)N-labeled Bm-YffB show that the protein binds reduced glutathione at a site adjacent to a region similar to the HX(3)CX(3)R catalytic sequence motif that is important for arsenic detoxification activity in the classical arsenate-reductase family of proteins. The latter observation supports the hypothesis that the ArsC-YffB family of proteins may function as glutathione-dependent thiol reductases. However, comparison of the structure of Bm-YffB with the structures of proteins from the classical ArsC family suggest that the mechanism and possibly the function of Bm-YffB and other related proteins (ArsC-YffB) may differ from those of the ArsC family of proteins.
Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis.,Buchko GW, Hewitt SN, Napuli AJ, Van Voorhis WC, Myler PJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1129-36. Epub 2011 Aug 16. PMID:21904062[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Buchko GW, Hewitt SN, Napuli AJ, Van Voorhis WC, Myler PJ. Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1129-36. Epub 2011 Aug 16. PMID:21904062 doi:10.1107/S1744309111006336
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