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7w6g
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==TKS-L190G mutant from Cannabis sativa in complex with lauroyl-CoA== | |
| + | <StructureSection load='7w6g' size='340' side='right'caption='[[7w6g]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7w6g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cannabis_sativa Cannabis sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W6G FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=8I9:~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl]+tridecanethioate'>8I9</scene>, <scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w6g OCA], [https://pdbe.org/7w6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w6g RCSB], [https://www.ebi.ac.uk/pdbsum/7w6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w6g ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/OLIS_CANSA OLIS_CANSA] Polyketide synthase responsible for olivetol biosynthesis. Catalyzes the first step in the cannabinoids biosynthetic pathway. The preferred substrate is hexanoyl-CoA, but accepts also CoA esters with C4 to C8 aliphatic side chains. When using malonyl-CoA and hexanoyl-CoA as substrates, produces undetermined compounds distinct form olivetol or olivetolic acid (PubMed:19581347) that could be hexanoyl triacetic acid lactone (HTAL) (PubMed:18323613) and pentyl diacetic acid lactone (PDAL) (PubMed:22802619). Produces olivetolic acid when acting in concert with olivetolic acid cyclase (OAC).<ref>PMID:18323613</ref> <ref>PMID:19454282</ref> <ref>PMID:19581347</ref> <ref>PMID:22802619</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The therapeutic effects of Delta(9)-tetrahydrocannabinol (Delta(9)-THC) can be enhanced by modifications of the pentyl moiety at C-3. The engineering of Cannabis sativa olivetolic acid cyclase and tetraketide synthase with F24I and L190G substitutions, respectively, in the biosynthesis of Delta(9)-THC serves as a platform for the generation of resorcylic acids up to 6-undecylresorcylic acid. These results provide insights into the development of THC analogs with chemically distinct acyl moieties at C-3. | ||
| - | + | Dual Engineering of Olivetolic Acid Cyclase and Tetraketide Synthase to Generate Longer Alkyl-Chain Olivetolic Acid Analogs.,Lee YE, Nakashima Y, Kodama T, Chen X, Morita H Org Lett. 2022 Jan 14;24(1):410-414. doi: 10.1021/acs.orglett.1c04089. Epub 2021 , Dec 23. PMID:34939812<ref>PMID:34939812</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7w6g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cannabis sativa]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Lee YE]] | ||
| + | [[Category: Morita H]] | ||
| + | [[Category: Nakashima Y]] | ||
Current revision
TKS-L190G mutant from Cannabis sativa in complex with lauroyl-CoA
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